Write about polymerase chain reaction testing, weather it pertain to a certain virus, bacteria genetics.

Expert Solution

Polymerase chain reaction testing or, PCR testing is used to study DNA in detail. It has two main reagents.

primers
DNA polymerase

The primer is a short single strand DNA fragment which provides a complementory sequence to target DNA. Two primers are used.

DNA polymerase are heat stable, for example, Taq polymerase

The first step: Two strands of DNA double helix are physically seperated at a high temperature. This is done in a process called Nucleic acid denaturation.

The second step: The temperature is lowered. Then the primers bind to complementory sequence of DNA. DNA polymerase comes and enzymatically assembles a new DNA strand from nucleotides. Nucleotides are the buiding blocks of DNA. The two DNA strands of the primers are used as templates for this to happen.

The third step: The DNA generated is used as template for replication. It sets a chain reaction in motion. Due to this chain reaction the original DNA template is exponentially amplified.

This amplified genetic material( DNA) is studied.

Numerous types of pathogen's genetic material such as that of different bacteria's and virus's genetic material can be studied by PCR. For example, HIV virus and pathogen causing tuberculosis can be studied. For detecting viral DNA, the primer used need to be specific to target DNA.

gladiator answered 2 years ago

Polymerase Chain Reaction

Explain briefly the PCR (Polymerase Chain Reaction) and its variants.

What is Polymerase Chain Reaction (PCR)? Brieflly, what led to the invention of Polymerase Chain Reaction?

Question of medical biology and genetics: What is PCR (polymerase chain reaction)? How does PCR work?...

Question of medical biology and genetics: What is PCR (polymerase chain reaction)? How does PCR work? What happens at each of pcr? Explain in details.

What are the optional requirements for Polymerase Chain Reaction (PCR)? And what are the factors that usually prove culprits in inhibition of Polymerase Chain Reaction (PCR)?

What are the important components for a successful Polymerase Chain Reaction (PCR)?

What are some of the biomedical applications of Polymerase Chain Reaction (PCR)?

33) List the three steps of a polymerase chain reaction (PCR).

33) List the three steps of a polymerase chain reaction (PCR). 34) The following events all occur during translation. List them in order.codon recognition of the second amino acid in the A site of the large ribosomal subunit small ribosomal subunit binds with mRNA and a methionine-tRNA the large ribosomal subunit binds to the small ribosomal subunit small ribosomal subunit scans mRNA for the AUG start codon translocation of tRNAs from P to E site and A to P site peptide bond formation between the first...

Question

Write about polymerase chain reaction testing, weather it pertain to a certain virus, bacteria genetics.

Solutions

Expert Solution

Related Solutions