What are the optional requirements for Polymerase Chain Reaction (PCR)? And what are the factors that usually prove culprits in inhibition of Polymerase Chain Reaction (PCR)?

What are the optional requirements for Polymerase Chain Reaction (PCR)?

And what are the factors that usually prove culprits in inhibition of Polymerase Chain Reaction (PCR)?

Expert Solution

The optional requirements for Polymerase Chain Reaction (PCR) include a number of additives and cosolvents to minimize mispriming and increase the polymerising efficiency (for GC rich templates). The cosolvents include formaamide, glycerol and additives include potassium glutamate, Ammonium sulphate.

Almost anything in excess can hamper the Polymerase Chain Reaction (PCR). Besides, usual suspects in PCR inbhibition are Proteinase K, Phenol, EDTA, Ionic detergents, Polyanions etc.

The optional requirements for Polymerase Chain Reaction include a number of additives and cosolvents and the usual suspects in PCR inbhibition are Proteinase K, Phenol, EDTA, Ionic detergents, Polyanions etc.

Shams answered 2 years ago

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What are the optional requirements for Polymerase Chain Reaction (PCR)? And what are the factors that usually prove culprits in inhibition of Polymerase Chain Reaction (PCR)?

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