Describe how you could separate and purify compound A from a
mixture of two neutral compounds...
Describe how you could separate and purify compound A from a
mixture of two neutral compounds (A and B) when A comprises 95% of
the total and B the other 5% of the total. Assume that A and B have
similar polarities.
Describe how you would separate a mixture containing benzoic
acid and a neutral substance, benzophenone. Both compounds are
insoluble in water and soluble in ether.
Describe how you could separate a mixture of cytochrome C,
ferritin and an enzyme X (MW 450 kDa and pI 8.4) using only one
chromatography column. Be sure to include the type of column, the
type of gel or resin, and the conditions to ensure elution ( and
separation) of the proteins.
briefly describe how the GST
tag could be utilized to purify the fusion
protein from a cell lysate?
please explain how the gst tag is used not general purification,
thank you.
You are attempting to purify a protein that has a pI of 9.0 from
a mixture of similarly sized proteins, and have access to different
purification columns. For this protein, you would select as your
first column for purification an:
(a) Anion exchange
(b) Cation exchange
(c) Gel filtration
(d) Heparin sulfate
(e) Ammonium sulfate
You are given a mixture containing two compounds, A and B. Both
compounds have a solubility of 1 g/ 100 mL of solvent at 20 °C and
16 g/ 100 mL of solvent at 100 °C. The sample is composed of 3.5 g
of A and 10 g of B. At 100 °C the entire sample just dissolves in a
minimum amount of solvent. The solution is cooled to 20 °C and
crystals are collected.
Calculate the composition of the...
Suppose you want to separate a mixture of the following
compounds: benzoic acid, m-cresol, p-aminobenzophenone, and
napthalene. Come up with a list of steps and chemicals needed to
most efficiently isolate all four compounds as solids with the
greatest purity possible.
Distillation is a technique that is to purify liquid compounds.
Explain how distillation is used to separate the target product
from any residual unreacted starting material.
[2.0 Marks]
Upon completion of the reaction (before filtration), an
additional 4.0 mL of 1.0 M NaOH/EtOH was added to the reaction.
What was the purpose of this step? Include a chemical reaction as
part of your answer. [1 Mark]
The term “side reactions” refers to any chemical reaction that
can occur that form...