What is conditioned medium? 5. What is a cell lysate? 6. Explain the difference between BSA...

What is conditioned medium?

5. What is a cell lysate?

6. Explain the difference between BSA and BCA.

7. What is the relationship between alpha secretase and TACE?

Expert Solution

ANS (5)- cell lysate---->cell lysis is the process of breaking down or denatures the cell wall and excrete out the organelles from the cell. the fluid present in the lysed cell containing the organelles is known as lysate.

Cell lysis can occur through these methods - the viral infection, osmotic mechanism, and enzymatic actions.

Enzymatic lysis--->It is the method in which cell wall is breakdown through the use of enzymes such as proteases.
Viral infection----->In this method the virus triggers the host cell mechanisms to break down the wall in order to release from the host cell after multiplication.
Osmotic mechanism----->It is the method in which cell is placed in high salt concentration and then in low salt concentration due to which water moves inside the cell and because of this cell got exploded by pressure exerted on the cell wall.

gladiator answered 2 years ago

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What is conditioned medium? 5. What is a cell lysate? 6. Explain the difference between BSA...

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