Question

QUESTION 1

Why is non-GMO (negative control) necessary for this experiment? Choose all that apply:

	A.	We didn’t really need this control
	B.	We need to ensure that PCR should not work for non-GM foods
	C.	We need a known non-GM sample to compare our test samples to
	D.	We need to verify that GM primers are specific, and don’t amplify other sequences

Answer 1

ANS. Genetic modification of food is a controversial issue due to debated concerns over health and environmental safety .This experiment demonstrates technical understanding of how the food DNA is identified,allowing educated decision making about the safety and potential of food.A certified non GMO (non genetically modified organisms)food product also puchased and used as negative control for to show what DNA separation looks like when no genetically modified. in this PCR technique was used to identify DNA of various food and plant products has been modified . and plymerase chain reaction (PCR) amplification is the common method for identification of GMO's(genitically modified organisms).it amplify the food DNA to determine the presence or absence of genetically modified sequences.the ability to detect the geneticaly altered DNA in food samples of interest allows for educated decision- making about the safety and potential dangerous of using genetiaclly modified organisms or 'GMO' in food samples.several controls are used in the procedure to ensure DNA is suscessfully extracted from the test foods(plant primers) and to provide known examples of both genetically modified and DNA and non genetically modified DNA.and the primers are specific to the transgenic DNA sequnece because the DNA is amplified many billion fold in this process,PCR is highly sensitive and specific GMO detection method.there are various ways to detect the GM DNA in a sample based on structure of foreign DNA sequence or trangene.