Where would you expect to find mutations that reduce the expression of a bacterial protein-coding gene?...

Where would you expect to find mutations that reduce the expression of a bacterial protein-coding gene? At least two different sites are possible

Expert Solution

Mutations are heritable changes that can occur spontaneously or can be induced. Mutations may occur usually during replication due to insertion or deletion of base or transition or transversion of base (purine to pyrimidine or vice versa). These replication errors are mostly corrected by proof reading activity of DNA polymerase enzyme. Sometimes, the mutation may be occurred because of change of base in DNA sequence is unnoticed. Such changes in DNA sequences may result in loss of function or gain of function of certain mutated gene. Usually, mutations that reduce the expression of protein encoding genes occur in promoters where the RNA polymerase is bound to initiate the transcription or in other regulatory genes such as operator or repressor that turn on or off the transcription of operon. Such mutations generally reduce the expression of protein encoding genes by turning off the transcription of operon.

gladiator answered 2 years ago

how do non protein coding sequences affect gene expression and protein activity

Would you be more likely to find single nucleotide polymorphism in the protein-coding or in the...

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Summarized Chapter 17 Gene expression: From Gene to Protein and Chapter 18 Regulation of Gene Expression....

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Generally speaking, which of the following mutations would most severely affect the protein coded for by a gene?

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Explain roles of non-coding RNA in the regulation of gene expression.

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When a cDNA carrying a protein coding region is inserted into a prokaryotic expression vector in...

When a cDNA carrying a protein coding region is inserted into a prokaryotic expression vector in order to produce the protein in bacteria, the orientation of the insert relative to the prokaryotic promoter must be known. Describe the use of a restriction endonuclease to determine the insert’s orientation in the vector (support your explanation with a drawing).

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