Question 3: Genetics and molecular biology of
sleep:
Reverse genetics (starting from gene
and progressing to phenotype, such as by cloning a gene and
creating a knockout mouse) and forward
genetics (starting from phenotype and progressing
towards the genes, such as by mutagenesis followed by mapping and
gene cloning) are experimental approaches that have been useful in
many fields including improved understanding of the circadian
pacemaker. Briefly discuss a couple successes of these approaches
applied towards understanding sleep, and then...
Explain what forward genetics and reverse genetics are?
Assume you have known the function of the gene A via genetic
analysis, design some experiments using both forward and reverse
genetics approaches to identify the gene X that may work in the
same pathway as the gene A.
Explain what forward genetics and reverse genetics are ?
Assume you have known the function of the gene A via genetic
analysis, design some experiments using both forward and reverse
genetics approaches to identify the gene X that may work in the
same pathway as the gene A .
8. Explain how the lacZ gene will be expressed when bacteria
with loss-of-function mutations in the lacI gene are in an
environment free of both lactose and glucose.
The idea is about genetic testing not about getting a specific answer.
In a genetics experiment of a pathway, loss of function in gene X or gene Y causes a blue phenotype, and loss of function in gene Z causes a green phenotype.
What experiments could you use to find the order and regulatory relationships of all the genes? Assume you have all the types of alleles you would want and explain what you would observe in your experiment if...
Name two examples of loss of function mutations
in the Pi transporter gene of E.coli? (If
references to scientific journals could also be included, it would
be highly appreciated)