Question

Describe how you would produce an anti-mouse IgG antibody in goats

Thank you in advance

Answer 1

Unconjugated Goat Anti-Mouse IgG Antibody binds to mouse primary antibodies for immunohistochemistry and many applications. The process is as below -

The anti-mouse Ig antibodies are prepared by hyperimmunizing Goat which produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis for further analysis.