Definition
The Papanicolaou test (Pap smear) is a non invasive cystologic test for early
detection of cervical cancer. The can also be used to detect
cancerous cells of the breast, lung, stomach, and renal system.
Here scrapes secretions from the patient’s cervix and spreads them
on a slide, which is sent to the laboratory for cystologic
analysis. Tests are advised yearly for women older than age 40, for
those in a high risk category, and for those who had a positive
test previously. If a Pap test is positive or suggest malignancy,
cervical biopsy can confirm the diagnosis.
Purpose
- To detect viral, fungal, and occasionally, parasitic
invasions.
- To assess response to chemotherapy and radiation therapy.
- Evaluate abnormal cervical changes (cervical dysplasia).
- Detect condyloma and endometriosis.
- To asess the patient’s response to chemotherapy and radiation
therapy.
Interfering factors
There are some factors which can alter the outcome of the
study
- Delay in fixing a specimen, destroy the effectiveness of the
stain and makes cytologic interpretation difficult.
- Improper collection site
- Use of lubricating jelly on the speculum that may affect the
viability of some organisms
- Specimen collection during normal menstruation can contaminate
the sample.
- Douching, using tampons, or having sexual intercourse within 24
hours before the procedure can wash away cellular deposits.
- Existing perineal infections that may interfere with hormonal
cytology.
Procedure
Patient Preparation
- Explain the procedure and obtain a
concent.
- Obtain the patient’s health history include parity, date of
last menstrual period, surgical status, contraceptive use, history
of bleeding, history of previous Pap smears, and history of
radiation or chemotherapy.
- Instruct the patient to avoid intercourse for 24 hours,
douching for 48 hours, and medication or creams for 1 week.
- Instruct the patient to empty her bladder just before the
test.
- During the procedure, she might experience a slight discomfort
however may feel some pain when the cervix is scraped.
- Tell the patient to lie on the examining table and to place her
heels in the stirrups and slide her buttocks to the edge of the
table.
Implementation
- The patient is assisted into the lithotomy position with her
feet in the stirrups.
- Unlubricated speculum is inserted into the cervix.
- The cervix is located.
- Secretions from the cervix and material from the endocervical
canal are collected with the help of endocervical brush and wooden
spatula.
- Specimens are spread on slides and immediately immersed in
fixative or sprayed with a fixative.
- Specimens are appropriately labeled with date and time. date of
last menses, collection site, and method.
- If lesions present, scrapings taken directly from the
lesion.
- The slides are preserved immediately for evaluation.
Nursing
Interventions
- Clean the perineal
area
- Help the patient to sit and ask
her to dress once the examination is completed.
- Provide sanitary napkin if
cervical bleeding occurs.
- Inform the patient about her next
Pap test.
Interpretation
Abnormal Results
- Cells with relatively large nuclei, only small amounts of
cytoplasm, abnormal nuclear chromatin patterns, and marked
variation in size, shape, and staining properties, with prominent
nucleoli, suggest malignancy.
- Benign tumour
- Dysplasia.
Normal Result
- If only normal cervical cells present.
Complications