Question
In: Physics
A. Determine the maximum absorbance wavelength for the dye (Amaranth) in nm (use internet or ask...
A. Determine the maximum absorbance wavelength for the dye (Amaranth) in nm (use internet or ask me for alternative method if you don't have regular access) and in a few sentences explain what is happening when inside the instrument when an absorbance spectra is being acquired. What are the units on the y-axis (ordinate) of the graph? What would be the problem with using a acrylic cell when the sample absorbs in the UV region of the EM spectrum? Explain IN DETAIL (including what glassware you would use, etc.) how you would prepare 10 standards of stock solution and do one calculation showing how the stock solution (3.0e-03 M) would be diluted to the most concentrated standard (3.0e-05 M) and what the purpose of using standards is when determining an unknown sample.
Solutions
genius_generous answered 2 years agoRelated Solutions
Substance H has maximum absorbance at 550 nm; Substance M has maximum absorbance at 650 nm....
Substance H has maximum absorbance at 550 nm; Substance
M has maximum absorbance at
650 nm. A standard solution of H has a concentration 74.2 ppm and
an absorbance of 0.972 at
500 nm and an absorbance of 0.276 at 650 nm. A standard solution of
M has a concentration
of 35.9 ppm and an absorbance of 0.102 at 500 nm and of 0.564 at
650 nm. A sample solution
that is a mixture of H and M has an...
why do you use a specific wavelength of light to record absorbance measurements for the dye?
why do you use a specific wavelength of light to record
absorbance measurements for the dye?
A) You are given a dye with an excitation maximum of 358 nm and an emission...
A) You are given a dye with an excitation maximum of 358 nm and
an emission maximum of 461 nm, and your fluorescence microscope has
fluorescence cubes with the filters below. Do you think you will
you be able to see any fluorescence from this dye using any of
these fluorescence cubes? Explain your answer. B) Does it
make a difference if the barrier filter is a band pass versus a
long pass? Why?
Filter cube Excitation (exciter) Emission (barrier)...
What is the wavelength of the maximum absorbance of the nanoparticles? To what colour of visible...
What is the wavelength of the maximum absorbance of the
nanoparticles? To what colour of visible light does this
correspond? Estimate a size for the gold nanoparticles formed.
For your indicator, what is the wavelength of maximum absorbance for the pH < 4.00 solution?...
For your indicator, what is the wavelength of maximum absorbance
for the pH < 4.00 solution? What is the wavelength of maximum
absorbance for the pH > 10.00 solution? What colors correspond
to these wavelengths? How do the observed colors of these solutions
relate to the colors at their absorbance maxima? This is the
procedure:
http://www3.chem21labs.com/labfiles/UofC_GL18_Lab.pdf?rf=5204
For your indicator, what is the wavelength of maximum absorbance for the pH < 4.00 solution?...
For your indicator, what is the wavelength of maximum absorbance
for the pH < 4.00 solution? What is the wavelength of maximum
absorbance for the pH > 10.00 solution? What colors correspond
to these wavelengths? How do the observed colors of these solutions
relate to the colors at their absorbance maxima?
This is the procedure:
http://www3.chem21labs.com/labfiles/UofC_GL18_Lab.pdf?rf=5204
Guanosine (Molar Mass = 151.13 g/mol) has a maximum absorbance of 275nm. At the maximum wavelength...
Guanosine (Molar Mass = 151.13 g/mol) has a maximum absorbance
of 275nm. At the maximum wavelength the molar absorption
coefficient is 8400 M^-1 cm^-1 and the path langth is 1.00 cm.
Using spectrophotometric data provided, find the mass of Guanosine
in this 25.0mL solution with a max A275 = 0.70. (The 275 is at the
bottom of A. )(Example, A^275 , but flipped to the bottom)
A plant pathologist used a spectrophotometer to measure the light absorbance of light (wavelength 500 nm)...
A plant pathologist used a spectrophotometer to measure the
light absorbance of light (wavelength 500 nm) by a protein
solution. The 26 measurements were as follows:
.121 .115 .135 .114 .099 .121 .107 .107 .103 .110 .106 .106 .098
.116 .148 .098 .121 .123 .124 .122 .106 .133 .114 .102 .123
.119
a. Calculate the sample mean, rounding appropriately.
b. Calculate the sample standard deviation, rounding
appropriately.
c. Find the 5-number summary for this data. (6 points) d.
Identify any...
Herb wants to know the wavelength of maximum excitation andemissionto the nearest 0.1 nm for a...
Herb wants to know the wavelength of maximum excitation
andemissionto the nearest 0.1 nm for a number of dyes
and also wants to get the lowest possible detection limit for each
dye (i.e. he wants to maximize signal). You want to
build the most sensitive spectrometer possible for a moderate cost.
How would you design a system to meet Herb’s needs. Be
specific about the components.
You know that certain blackbody emits maximum radiation at 500 nm. How will the wavelength change...
You
know that certain blackbody emits maximum radiation at 500 nm. How
will the wavelength change if you decrease the temperature of the
blackbody by 1000K? Where will the wavelength and frequency be
now?
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