Guanosine (Molar Mass = 151.13 g/mol) has a maximum absorbance of 275nm. At the maximum wavelength...

Guanosine (Molar Mass = 151.13 g/mol) has a maximum absorbance of 275nm. At the maximum wavelength the molar absorption coefficient is 8400 M^-1 cm^-1 and the path langth is 1.00 cm. Using spectrophotometric data provided, find the mass of Guanosine in this 25.0mL solution with a max A275 = 0.70. (The 275 is at the bottom of A. )(Example, A^275 , but flipped to the bottom)

Expert Solution

A₂₇₅ = absorbance = 0.70 molar absorption coefficient = 8400 M^-1 cm^-1

path langth l= 1.0 cm Concentration c= ?

According to Beer's law A =lc

0.70 = 8400 x 1 x c c = 0.70/8400 = 8.33 x 10^-5 M

Concentration of Guanosine = 8.33 x 10^-5 M

Volume of Guanosine = 25.0 ml = 25/1000 = 0.025 lit

Molar Mass of Guanosine = 151.13 g/mol)

mass of Guanosine in 25.0mL solution = molarity x molar mass x vol in lit

= 8.33 x 10^-5 x 151.13 x 0.025 = 3.15 x 10^-4 grams

queen_honey_blossom answered 2 years ago

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