Question
In: Physics
The resolution of microscopy limits by the diffraction barrier; however, super-resolution microscopy is capable of imaging...
The resolution of microscopy limits by the diffraction barrier; however, super-resolution microscopy is capable of imaging below the diffraction limit. Explain the basic principle of any super-resolution using your own words how it is possible to resolve below the diffraction limit.
Solutions
genius_generous answered 1 year agoRelated Solutions
Discuss two different criteria for the limits or resolution. What are the different resolution thresholds?
Discuss two different criteria for the limits or resolution.
What are the different resolution thresholds?
Explain what the diffraction limit is and how it is connected to the spatial resolution of...
Explain what the diffraction limit is and how it is connected to
the spatial resolution of an optical system. Is it possible to
resolve features smaller than the diffraction limit? Can you
resolve 100 nm features using an optical microscope? How about 100
pm?
Explain what the diffraction limit is and how it is connected to the spatial resolution of...
Explain what the diffraction limit is and how it is connected to
the spatial resolution of an optical system. Is it possible to
resolve features smaller than the diffraction limit? Can you
resolve 100 nm features using an optical microscope? How about 100
pm?
what is the difference between x-ray imaging and the x-ray diffraction?
what is the difference between x-ray imaging and the x-ray
diffraction?
Compare and contrast these aspects below of electron microscopy with standard light imaging. (diffs/similarities)
Compare and contrast these aspects below of electron microscopy with standard light imaging. (diffs/similarities)
a. Lens system (purpose and mechanism)b. Information that can be concluded from acquired images apart from structurec. Sample preparation (fixed, mounting for imaging)
Briefly explain the following terms in a few sentences: a. Resolution of optical microscopy b. Langmuir...
Briefly explain the following terms in a few sentences:
a. Resolution of optical microscopy
b. Langmuir versus Langmuir-Blodgett (LB) film
c. The wavelength of electrons at an acceleration voltage of 100
kV
d. Chemically amplified photoresist
e. Elastic versus inelastic scattering
f. Capillary force
Transmission Electron Microscopy a).Explain how bright field and dark field imaging is obtained in a TEM....
Transmission Electron Microscopy
a).Explain how bright field and dark field imaging is obtained
in a TEM.
b).What is the difference between bright/dark field imaging and
high resolution TEM?
c).Describe why you see contrast in TEM images, e.g. the origin
of the contrast in bright field imaging, dark field imaging,
mass-thickness contrast, z-contrast.
Using the paper, “Multiparametric Atomic Force Microscopy Imaging of Biomolecular and Cellular Systems”, describe what is...
Using the paper, “Multiparametric Atomic Force Microscopy
Imaging of Biomolecular and Cellular Systems”, describe what is
meant by “Multiparametric AFM”. Describe the impact and
advantages of this approach. (Acc. Chem. Res. 2017, 50,
924−931)
What is the diffraction-limited optical resolution of the human eye? (feel free to find the geometrical...
What is the diffraction-limited optical resolution of the human
eye? (feel free to find the geometrical and optical properties of
the eye using literature search)
B._____ Determine the high-resolution structure of a mitochondrion in cross section Atomic force microscopy (AFM) Light...
B._____ Determine the high-resolution structure of a
mitochondrion in cross section
Atomic force microscopy (AFM)
Light microscopy, standard
Light microscopy with differential interference contrast
(DIC)
Scanning electron microscopy (SEM)
Transmission electron microscopy (TEM)
Fluorescence microscopy, standard
Laser scanning confocal microscopy (LSCM)
Live cell imaging of GFP-tagged protein
Fluorescence resonance energy transfer
(FRET)
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