In: Biology
what enzyme is necessary for the formation and transfer of the peptide bond to the next amino acid brought by the tRNA, and what enzyme is responsible for the charging of the tRNA with another, correct, amino acid?
Transfer rna or tRNA is involved in transfer of the nucleus acid message into amino acids which are the individual units of protein . At its end it has an anticodon loop which pairs with mRNA which specifies a particular amino acid while the other end has the amino acid attached via ester linkage . Protein synthesis happens when a charged tRNA , mRNA and small and large ribosomal units come together to form an intitation c complex which has a P site ( peptidyl binding) and an A site ( aminoacyl binding). The first tRNA to bind is initiator tRNA and the first amino acid is methionine . Elongation of the polypeptide chain occurs when anticodon of the second charged tRNA binds to next mRNA codon in the A site of initiator c complex.
P and A sites are close and this causes the carboxy terminus at P site to form a stable peptide bond with the nucleophilic amino terminus at A site . This formation of the peptide bond is catalysed by peptidyl transferase . It's a ribozyme located in the larger ribosomal unit .
The tRNA which has an amino acid attached to it is called a charged tRNA .The enzyme responsible for this aminoacyl tRNA synthetase.