Question

Briefly explain how a cell line overexpressing a specific protein can be constructed using CRISPR

Answer 1

ANSWER ) Targeted gene upregulation using the CRISPR/Cas9 system can be done by homology directed repair mediated insertion of a transcriptional promoting element . This powerful platform employs a ubiquitous chromatin opening element, promoter, and translationally relevant EGFR reporter to drive endogenous gene overexpression. One of the research this novel engineering methodology allowed for high level COL7A1 expression in allogeneic umbilical cord blood hematopoietic stem cells and peripheral blood T-cells . The COL7A1 gene provides instructions for making a protein called pro-α1(VII) chain that is used to assemble a larger protein called type VII collagen. Collagens are a family of proteins that strengthen and support connective tissues, such as skin, bone, tendons, and ligaments, throughout the body. In particular, type VII collagen plays an essential role in strengthening and stabilizing the skin. Thus CRISPER/Cas9 is a robust and user-friendly platform is readily deployable for targeted gene overexpression in multiple applications.