Question
In: Biology
Compare bacterial chromosome and plasmids based on the following criteria: Criteria Chromosome Plasmid Number --------------------------------...
Compare bacterial chromosome and plasmids based on the following
criteria:
Criteria Chromosome Plasmid
Number -------------------------------- -- --------------------------
Type of gene present ------------------------------------------ ------------------------------------------
Purpose /function ----------------------------------------------
----------------------------------------
Solutions
Expert Solution
|
Criteria |
Bacterial chromosome |
Bacterial plasmid |
|
Number |
|
|
|
Type of genes present |
antibiotic resistance genes, transgenes and reporter genes |
|
|
Purpose/function |
|
|
gladiator answered 2 years agoRelated Solutions
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Chromosome Chromosome Locus Number Locus Number &nb
Chromosome
Chromosome
Locus
Number Locus
Number
TPOX
2
TH01
11
D3S1358
3
VWA
12
FGA
4
D13S317
13
D5S818
5
D16S539
16
CSF1PO
5
D18S51
18
D7S820
7
D21S11
21
D8S1179
8
Table 1.
Locus
Alleles
Frequency
Locus RMP
TPOX
8
12
0.535
0.041
TH01
10
10
0.008
D3S1358
16
17
0.222
0.222
FGA
21
23
0.0185
0.134
CSF1PO
11
13
0.301
0.096
D8S51
14
19
0.137
0.038
D21S11
28
29
0.159
0.195
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1) You wish to clone a gene into a bacterial plasmid for the production of the...
1) You wish to clone a gene into a bacterial plasmid for the
production of the E protein to generate antibodies, use in vaccine
production, and for biochemical analysis.
A. What expression plasmid will you use and why?
B. What primers to facilitate cloning into this expression
vector?
C. What essential features (DNA sequence elements) must the
plasmid have to ensure:
i. Plasmid maintenance/amplification in bacteria
ii. Selection of cells transformed with your plasmid
iii. Controlled/inducible expression of Gene E...
The structure of the bacterial chromosome remains the same during bacterial cell growth. Do you agree?...
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R-plasmids are plasmid vectors which encode what type of genes? (user thinks genes for replicases) Homologous...
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(user thinks genes for replicases)
Homologous recombination results in a protein with which
attributes? (user thinks it a protein with amino acid sequence)
Transduction is the process best described as? (the uptake of
DNA by infection)
Ribosomal RNA sequencing is utilized to create phylogenetic
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5.) What domian contain Chromosomes...
Experiment 2: Cloning a DNA Fragment to a Bacterially-Derived Plasmid Vector Bacteria frequently contain extrachromosomal plasmids,...
Experiment 2: Cloning a DNA Fragment to a Bacterially-Derived
Plasmid Vector
Bacteria frequently contain extrachromosomal plasmids, which are
circular DNA genetic elements that are self-replicating. Plasmids
are typically not necessary for the bacteria's survival but can
sometimes confer a growth advantage to the bacteria. Plasmids can
be transferred between bacteria and recombinant DNA technology
makes use of this feature to introduce “foreign” genes into
bacteria and use the bacteria as a cell “factory” to produce the
gene. Genes are inserted...
2) Please explain how to purify plasmid DNA from bacterial cells.
2) Please explain how to purify plasmid DNA from bacterial
cells.
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