Question

In: Chemistry

4. The enzyme alcohol dehydrogenase catalyzes the oxidation of ethyl alcohol by NAD+ to give acetaldehyde...

4. The enzyme alcohol dehydrogenase catalyzes the oxidation of ethyl alcohol by NAD+ to give acetaldehyde plus NADH and a proton:

CH3CH2OH + NAD+                  CH3CHO + NADH + H+

The rate of this reaction can be measured by following the change in pH. The reaction is run in 1 mL 10 mM TRIS buffer at pH 8.6. If the pH of the reaction solution falls to 8.4 after ten minutes, what is the rate of alcohol oxidation, expressed as nanomoles of ethanol oxidized per mL per sec of reaction mixture?

Solutions

Expert Solution


Related Solutions

The human pathway for metabolizing alcohol starts with the enzyme alcohol dehydrogenase, which catalyzes the conversion...
The human pathway for metabolizing alcohol starts with the enzyme alcohol dehydrogenase, which catalyzes the conversion of ethanol (C2H5OH) to acetaldehyde (CH3CHO). This is followed by the aldehyde dehydrogenase 2 (ALDH2, the enzyme of interest in this problem), which catalyzes the conversion of acetaldehyde and HS-CoA to acetyl-CoA (CH3CO–S–CoA). The TCA cycle starts and oxidizes the acetyl-CoA to CO2. Draw two diagrams of this pathway—one for an individual without AFS and another for an individual with AFS. How ALDH2 deficiency...
What is alcohol dehydrogenase (ADH) and nicotinamide adenine dinucleotide (NAD)? What role do they play in...
What is alcohol dehydrogenase (ADH) and nicotinamide adenine dinucleotide (NAD)? What role do they play in regards to the conversion of alcohol?
The enzyme known as alcohol dehydrogenase is capable of oxidizing ethanol, methanol and other hydroxyl group...
The enzyme known as alcohol dehydrogenase is capable of oxidizing ethanol, methanol and other hydroxyl group containing organic compounds into their corresponding aldehydes. Such an enzyme with ___ catalyzes the reaction of structurally related substances. Select one: a. absolute specificity b. relative specificity c. stereospecificity d. no specificity
(THREE PARTS) Ethyl alcohol is formed by enzyme action on sugars and starches during fermentation. The...
(THREE PARTS) Ethyl alcohol is formed by enzyme action on sugars and starches during fermentation. The UNBALANCED equation is as follows: C6H12O6 ----> CO2 + C2H6O A) Write the balanced chemical equation. B) If the density of ethyl alcohol is 0.789 g/mL, how many gallons of ethanol alcohol can be produced by fermentation of 100.0 lb of sugar? C) How many gallons of ethyl alcohol was produced from the fermentation of 100.0 lbs of sugar if your homemade still only...
The enzyme glucose oxidase isolated from the mold Penicillium notatum catalyzes the oxidation of β-D-glucose to...
The enzyme glucose oxidase isolated from the mold Penicillium notatum catalyzes the oxidation of β-D-glucose to D-glucono-δ-lactone. This enzyme is highly specific for the β anomer of glucose and does not affect the α anomer. In spite of this specificity, the reaction catalyzed by glucose oxidase is commonly used in a clinical assay for total blood glucose –that is, for solutions consisting of a mixture of β-D-glucose and α-D-glucose. What makes it possible for this test to measure total blood...
ADVERTISEMENT
ADVERTISEMENT
ADVERTISEMENT