Question

How are the DNA polymerases used in nature (DNA replication) and sequencing different?

What other steps in DNA sequencing are different than prokaryotic DNA replication?

Answer 1

(a)DNA polymerase replication:

-DNApolymerase are enzymes that synthesize DNA molecules from deoxyribonucleotides, the building blocks of DNA. These enzymes are essential for DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase "reads" the existing DNA strands to create two new strands that match the existing ones.

DNA polymerase adds nucleotides to the 3'- end of a DNA strand, one nucleotide at a time.

DNA sequencing:-is the process of determining the precise order of nucleotideswithin a DNA molecule. It includes any method or technology that is used to determine the order of the four bases—adenine, guanine, cytosine, and thymine—in a strand of DNA.

structure of DNA has four bases: thymine (T), adenine (A), cytosine (C), and guanine (G). DNA sequencing is the determination of the physical order of these bases in a molecule of DNA.

(b)