How are the DNA polymerases used in nature (DNA replication) and
sequencing different?
What other steps in DNA sequencing are different than
prokaryotic DNA replication?
C.K. Tang used three different privatisation methods. Explain
how these different methods work and the pros and cons of these
different methods from the viewpoints of the shareholder(s) wanting
to take a company private versus minority shareholders who may
prefer that the company remain listed.
3.Briefly describe the concepts of DNA sequencing. How it has
evolved over the time? What is the current status of sequencing and
pick a method to outline the principles as well as reasons you
think it is the best?
4.What do you understand by “omics” revolution? Why do we need
different “omics” to understand genes and proteins functions? Pick
a specific omics and elaborate how it may help to understand
fundamental levels as well as helping us to live a...
Different chemical precursors are used in the synthesis of
hormones.
a) What are the 3 main categories of hormones in a chemical
sense?
b) What advantages does this different chemical nature of
hormones provide in terms of how hormones act at their target
cells?
Different chemical precursors are used in the synthesis of
hormones.
a) What are the 3 main categories of hormones in a chemical
sense?
b) What advantages does this different chemical nature of
hormones provide in terms of how hormones act at their target
cells?
************ Please leave a detailed description to part 2.
Thank you :)
The DNA polymerases used over many years for Sanger
dideoxynucleotide termination sequencing of DNA have had one common
feature: they all were intentionally chosen to lack 3’à 5’
exonuclease activity, the proofreading function that many DNA
polymerases carry to maintain high fidelity. Why would having
proofreading exonuclease activity within the polymerase be
detrimental to the Sanger sequencing technique? If you were to
substitute a polymerase with proofreading capability, how might
that affect the distribution of the bands of DNA produced...
Illumina sequencing is a ‘Next Generation Sequencing’
technology. Explain the flow of how the Illumina sequencing
technique workk. (1.5 pts)
There are several commercially available DNA polymerases that
are used in sequencing. Two of these DNA polymerases are
Taq and Q5. Explain some of the similarities and
differences between these two polymerases. (1 pt)