1. name and describe the 3 Cis-acting regions of a prokaryotic gene that aid prediction computer...

1. name and describe the 3 Cis-acting regions of a prokaryotic gene that aid prediction computer programs, such as BLAST in identify genes.

2. List at least 3 reason why finding genes in eukaryotes is more complicated than prokaryotes. Think about overall structure , cis-acting genetic elements

3. What is gene annotation?

Expert Solution

1) Cis acting regions: DNA sequences usually 100-1000bp in the vicinity of the structural portion of a gene to which transcriptional factor proteins binds which are required for gene expression.

They are labeled as cis because they are typically located on the same DNA strand as the genes they control as opposed to trans, which refers to effects on genes not located on the same strand or farther away, such as transcription factors (RNA ploymerase)

Cis-regulatory modules typically divided into three classes namely enhancers ( regulate gene expression positively) insulators ( works indirectly by interacting with other nearby cis-regulatory regions) and silencers (turn off expression of genes)

Answer 2)

a) Much of the complexity of eukaryotic genomes results from the abundance of several different types of noncoding sequences.

b) Presence of complex and not well understand promoter and other gene regulatory elements

C) Presence of complex splicing mechansim for removal of Introns

Answer 3)Gene Annotation is the process by which information about raw DNA sequences is added to the genome databases. This involves describing different regions of the code and identifying which regions can be called genes.

gladiator answered 2 years ago

Compare the cis acting elements of prokaryotic and eukaryotic transcriptional regulation.

For each of the following, indicate if it is cis-acting or trans-acting A regulatory gene that...

For each of the following, indicate if it is cis-acting or trans-acting A regulatory gene that codes for a transcription factor _______________ A promoter _______________ An operator _________________ An enhancer _________________

List 3 different types of cis-acting elements that influence eukaryotic gene expression and briefly describe their function.

The necessary components for gene expression are often described as either cis-acting elements (“acting from the...

The necessary components for gene expression are often described as either cis-acting elements (“acting from the same molecule”) or trans-acting factors (“acting from a different molecule”). Cis-acting elements are typically sequences that are required for expression. Trans-acting factors are typically proteins that recognize and bind cis-acting sequences to control or facilitate gene expression. For each of the cis-acting elements below, Promotor and Start Codon,list a trans-acting factor involved in its recognition and describe the factor’s normal function

Describe the difference between cis-acting and trans-acting elements. Also list whether the following elements are cis- or trans-acting:

Describe the difference between cis-acting and trans-acting elements. Also list whether the following elements are cis- or trans-acting: A) β-galactosidase B) Operator site C) LacI repressor protein D) Lactose E) Permease F) Promoter site

4. The necessary components for gene expression are often described as either cis-acting elements (“acting from...

4. The necessary components for gene expression are often described as either cis-acting elements (“acting from the same molecule”) or trans-acting factors (“acting from a different molecule”). Cis-acting elements are typically sequences that are required for expression. Trans-acting factors are typically proteins that recognize and bind cis-acting sequences to control or facilitate gene expression. For each of the trans-acting factors below, describe their enzymatic activity and one of their cis-acting target sequences. [8 pts; 4 pts each] 4a. RNA Polymerase...

Discuss eukaryotic gene regulation including the cis-acting elements and the major groups of trans-acting factors.

Discuss eukaryotic gene regulation including the cis-acting elements and the major groups of trans-acting factors. Be sure to include the relative distances from the gene and the sequence characteristics of the major elements.

Describe the difference between cis-acting and trans-acting elements. Also list whether the following elements are cis-...

Describe the difference between cis-acting and trans-acting elements. Also list whether the following elements are cis- or trans-acting: A) Beta-galactosidase B) Operator site C) LacI repressor protein D) Lactose E) Permease F) Promoter site

Of all the cis-acting elements associated with a gene, which is the most important? Why?

a promoter ___ a cis acting site 1. is 2. is not

Question