1. If a mutant sigma factor binds very tightly to RNA polymerase, how might that affect...

1.

If a mutant sigma factor binds very tightly to RNA polymerase, how might that affect transcription?

It would inhibit the initiation phase of transcription

It would inhibit the transition between initiation and elongation.

It would inhibit the rate of elongation.

It would enhance termination.

2. In bacteria, what type of interaction causes an mRNA to bind to the small ribosomal subunit?

The mRNA has a 5’ cap that is recognized by cap proteins that cause it to bind to the small ribosomal subunit.

The mRNA has a Shine-Dalgarno sequence that is complementary to the portion of RNA in the small ribosomal subunit.

The mRNA is recognized by initiation factors that bind GTP and carry the mRNA to the small ribosomal subunit.

The mRNA is recognized by the large ribosomal subunit that docks onto the small ribosomal subunit.

Expert Solution

it would inhibit the transition from initiation and elongation. because, sigma factor helps RNAP to recognise the promoter, this helps in intiation of transcription. but, then sigma factor is released so that the enzyme could proceed further.
the mRNA has shine dalgarno sequence which interacts with the 16s subnit of the 30s ribosome.

gladiator answered 2 years ago

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