#1. Heparin is a polyanionic polysaccharide that blocks initiation by RNA polymerase by virtue of its...

#1. Heparin is a polyanionic polysaccharide that blocks initiation by RNA polymerase by virtue of its binding to double stranded DNA. However, Heparin inhibits only when added before the onset of transcription, and not if added after transcription begins. Explain the difference.

#2. Describe how RNA Polymerase backtracking could function to increase the fidelity of transcription.

Expert Solution

1. Heparin is highly negatively charged. It binds to the lysine and Arginine residues of RNA pol which causes it to change its confirmation and prevents its binding to the DNA. However, once bound to the DNA, it no longer has exposed positively charged residues so that heparin could bind to them.

2. RNA pol backtracks and then checks the fidely of the newly synthesized transcript. In backtracked state the wrongly incorporated ribonucleotide is readily cleaved in the presence of elongation factor IIS. Thus, after this the new and correct ribonucleotide is incorporated.

gladiator answered 2 years ago

What aspect of heparin allows it to bind effectively to the RNA polymerase active site?

Describe the initiation, elongation and termination phases of transcription. Include the following terms: RNA polymerase, promoter...

Describe the initiation, elongation and termination phases of transcription. Include the following terms: RNA polymerase, promoter DNA sequence, DNA template strand, terminator DNA sequence.

compare between polysaccharide : 1.heparin 2.hyaluronic acid

compare between polysaccharide :1.heparin2.hyaluronic acid3.chitinfrom the*name of sugar*homo polysaccharides or hetero polysaccharides*the unit and structure*the function (Where is it and why?)*Solubility in water and why?*reducing or non reducing and why?

Compare and contrast the properties of DNA polymerase and RNA polymerase.

Compare and contrast the properties of DNA polymerase and RNA polymerase. Drag the appropriate items to their respective bins.

Cellular RNA polymerase cannot transcribe anRNA template. So, where does the RNA polymerase activity come from...

Cellular RNA polymerase cannot transcribe anRNA template. So, where does the RNA polymerase activity come from in the case of: A ssRNA virus with a + sense genome? A ssRNA virus with a – sense genome?

6. Compare the structure and function of a typical DNA polymerase and a typical RNA polymerase.

6. Compare the structure and function of a typical DNA polymerase and a typical RNA polymerase.7. What are the post-transcriptional modifications that the pre-mRNA is subjected to in eukaryotes? Explain by giving the role(s) of each modification.8. Highlight the general characteristics of a mature mRNA in comparison with rRNA or tRNA

The enzyme required for transcription is * RNA polymerase Restriction enzymes Splicesome DNA polymerase

Discuss the structure of promoter for RNA Polymerase II

How is the polysaccharide heparin different from the glucose polysaccharides? In contrast to the glucose polysaccharides...

How is the polysaccharide heparin different from the glucose polysaccharides? In contrast to the glucose polysaccharides that contain ______ as the repeating unit, heparin contains ______ as the repeating unit. The monosaccharides in _______ are charged, whereas _________ is not. Choices: Three monosaccharides, heparin, four monosaccharides, two monosaccharides, glucose

9.) During the process of DNA replication, both DNA polymerase and RNA polymerase are involved in...

9.) During the process of DNA replication, both DNA polymerase and RNA polymerase are involved in eventually creating the new daughter strand of DNA. T or F 10.) Replication begins at the origin of replication or ORI and transcription begins at the first codon for methionine. T or F 11.) The process of mapping, sequencing and analysis of proteins is called genomics. T or F 12.) The heavy chain constant domains of a given immunoglobulin molecule define its class. T...

Question