Question

Dr. Nerdman wants to inhibit a cell from producing Protein A. Which of the following experimental treatments could he use to accomplish his goal?

a) Expose the cell to a chemical at binds to the promoter of the gene that encodes Protein A

b) Expose the cell to a chemical that inhibits the large and small ribosomal subunits from binding

c) Expose the cell to a chemical that degrades all mRNA

d) Expose the cell to a chemical that degrades all tRNA

e) All of the above

Answer 1

e) All of the above

a) Expose the cell to a chemical at binds to the promoter of the gene that encodes Protein A ----> Pre-transcriptional inhibition of protein synthesis

b) Expose the cell to a chemical that inhibits the large and small ribosomal subunits from binding ---> Post-transcriptional inhibition of protein synthesis.

c) Expose the cell to a chemical that degrades all mRNA ----> Post-transcriptional inhibition of protein synthesis.

d) Expose the cell to a chemical that degrades all tRNA ----> Post-transcriptional inhibition of protein synthesis.

The process of formation of mRNA from DNA is known as transcription. After mRNA formation, the extent to which the mRNA is translated into proteins is again regulated by some sort of mechanism. Modulation of the mRNA processing such as capping, poly (A) addition, and splicing, modulation of sequence specific nuclear export rates, etc. are observed in the eukaryotic cells as a part of post transcriptional regulation.