In: Biology
which of the following is not involved in the initiation of translation in bacteria
a- mRNA
b- tRNA carrying N-formylmethionine that will occupy the P site
c- GTP and initiation factors
d- small and large ribosomal subunits
e- tRNA carrying the next amino acid that will occupy the A site
Answer : The correct answer is E (tRNA carrying the next amino acid that will occupy the A site). This occurs during the elongation step of protein synthesis.
Explanation : During elongation the nascent polypeptide is lengthened by covalent attachment of successive amino acid units, each carried to the ribosome and correctly positioned by its tRNA, which base-pairs to its corresponding codon in the mRNA. Elongation requires cytosolic proteins known as elongation factors. The binding of each incoming aminoacyl-tRNA and the movement of the ribosome along the mRNA are facilitated by the hydrolysis of GTP as each residue is added to the growing polypeptide.