In: Chemistry
What effect do you think it would have if KBr was added to any of the reactions that gave a precipitate? Note that the bromide ion is a better nucleophile than the chloride ion
organic molecule are present in solution they are acts as non electrolyte. The KBr is a salt which undergo dissociation to poduce the K+ and Br- the are acting as a electrolyte they creates the electrolyte and non electrolyte in the solution then decreases the solubility of organic molecule that creates the pricipitate in the solution.
Salting out is a purification method that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength. Salting out is typically, but not limited to, the precipitation of large biomolecules such as proteins.
In contrast to salting in, salting out occurs in aqueous solutions of high ionic strength that reduce the molecule's solubility causing certain proteins to precipitate. Ideally, the type of salt being used and the concentration of the salt can be varied to selectively precipitate a the molecule. In reality, salting out is an effective means for initial molecule purification, but lacks the ability for precise isolation of a specific protein.