Beer-Lambert law relates the attenuation of light to the properties of the samples in UV-Vis spectrometer;...

Beer-Lambert law relates the attenuation of light to the properties of the samples in UV-Vis spectrometer; however, in general, the experimental results do not follow the Beer’s law. What are the limitations of Beer’s law?

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Expert Solution

According to beer lamberts law

Absorbance

is - molar absorption coefficient C is the cincentration and l is the length of light path

Absorbance is directly preption to concentration.

A can also written as

hence this also can written as

The limitations of this law is

1) This is law is derived for dilute solutions. At higher concentration molecular interaction can occour and deviations can happen.

2) effect of scattering of the light from samples is not accounted.

3) Fluoresent and phosphoresent samples makes deviation from this law.

4) source should be monochromatic.

5) Effect of refractive index of light absorption was not inclued.

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genius_generous answered 1 year ago

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