Question

In: Physics

Beer-Lambert law relates the attenuation of light to the properties of the samples in UV-Vis spectrometer;...

Beer-Lambert law relates the attenuation of light to the properties of the samples in UV-Vis spectrometer; however, in general, the experimental results do not follow the Beer’s law. What are the limitations of Beer’s law?

Expert Solution

According to beer lamberts law

Absorbance

is - molar absorption coefficient C is the cincentration and l is the length of light path

Absorbance is directly preption to concentration.

A can also written as

hence this also can written as

The limitations of this law is

1) This is law is derived for dilute solutions. At higher concentration molecular interaction can occour and deviations can happen.

2) effect of scattering of the light from samples is not accounted.

3) Fluoresent and phosphoresent samples makes deviation from this law.

4) source should be monochromatic.

5) Effect of refractive index of light absorption was not inclued.

(* If you like the answer please give a positive feed back thank you*)

genius_generous answered 2 years ago

Write down the Beer-Lambert law as it applies to neutron radiography. Calculate the attenuation coefficient of...

Write down the Beer-Lambert law as it applies to neutron radiography. Calculate the attenuation coefficient of hydrogen and iron for thermal neutrons and for 100 KeV X-rays (four attenuation coefficients in total). Show all steps and quote your resources. How will neutron beam attenuation differ between these two materials and between the two types of radiation?

What are the two most important fundamental limitations of the Beer-Lambert Law?

using beer lambert law . determine which of the following is either an independent variable or...

using beer lambert law . determine which of the following is either an independent variable or dependent variable. concentration of photons vs absorption absorption of varies colors

Use the following information and the Beer-Lambert law to determine the mass of the lycopene extracted...

Use the following information and the Beer-Lambert law to determine the mass of the lycopene extracted and the mass of lycopene per 100 grams of tomato paste. 2mL of lycopene was extracted from 8 grams of tomato pase. This was then diluted to 5mL. 1ml of the diluted solution was withdrawn and then further diluted to 9mL. The UV-vis absorbance at 502nm was determined to be 0.162. The cell path was 1cm.

Explain how do we construct a standard curve in the UV/Vis spectrometer lab,what do we use...

Explain how do we construct a standard curve in the UV/Vis spectrometer lab,what do we use the standard curve for?

Use the Beer-Lambert law to explain how a colorimetric assay works in the spectrophotometer. In your...

Use the Beer-Lambert law to explain how a colorimetric assay works in the spectrophotometer. In your answer, define the following terms; chromophore, extinction coefficient, absorbance, transmission and reagent blank. Most of the spectrophotometers in the teaching lab emit light of wavelengths between 400-700nm. However some spectrophotometers also emit light between 200-400nm. Why is this extended range useful? Provide a specific example. How can you use a spectrophotometer to perform a qualitative analysis of a substance?

This is for the verification of beer-lambert law experiment. Volume of copper sulphate solution /mL Volume...

This is for the verification of beer-lambert law experiment. Volume of copper sulphate solution /mL Volume of Concentrate d ammonia /mL Final volume of solution /mL [CuSO4]/m ol dm-3 [CuSO4]/pp m [CuSO4]/mg dm-3 Transmit tance /% Absorbance 0 25 25 101.5 0.006 1 24 25 95.9 0.018 2 23 25 89.9 0.046 3 22 24 86.7 0.062 4 21 25 81.4 0.090 Can someone show one sample example of how to determine the three ways the following concentrations can...

Explain the relationship between light absorbance and solution concentration for a solution which obeys the Beer-Lambert...

Explain the relationship between light absorbance and solution concentration for a solution which obeys the Beer-Lambert law.

I need to use Beer's Law to determine a concentration, yet my book states "The Beer-Lambert...

I need to use Beer's Law to determine a concentration, yet my book states "The Beer-Lambert Law is: A = ε0 b c The terms ε0 (extinction coefficient) and b (path length) are constant under the conditions of this experiment, allowing the further simplification Absorbance α (is directly proportional to) concentration." Would this not then mean that my number for absorbance is always equal to the number of concentration? I know I need to use y = mx + b,...

1. What does the Beer-Lambert Law allow you to do? 2. Under what conditions is the...

1. What does the Beer-Lambert Law allow you to do? 2. Under what conditions is the Beer-Lambert Law valid? 3. Under what conditions is the Beer-Lambert Law not valid?