Question

B._____ Determine the high-resolution structure of a mitochondrion in cross section

1. Atomic force microscopy (AFM)

2. Light microscopy, standard

3. Light microscopy with differential interference contrast (DIC)

4. Scanning electron microscopy (SEM)

5. Transmission electron microscopy (TEM)

6. Fluorescence microscopy, standard

7. Laser scanning confocal microscopy (LSCM)

8. Live cell imaging of GFP-tagged protein

9. Fluorescence resonance energy transfer (FRET)

Answer 1

Ans.

• Mitochondria are the powerhouse of cells which produce ATP as the source of energy.
• It was among the first several subcellular organs which were studied with the help of electron microscopy.
• Depending upon the cross-section taken, the appearance of electron micrographs of mitochondria may vary.
• Transmission electron microscopy (TEM) produces a high resolution 3-d structure of a mitochondrian sample during cross-section.
• TEM takes snapshots of mitchondrian micrograph at several different angles and planes to generate a 3-D representation by compiling these images.
• Several features of active mitochondria have been elucidated through electron microscopy such as;

1. Inner mitochondrial membrane and cristae are in continution.
2. Width of intermembrane space is consistent along the entire mitochondria.
3. In an active mitochondria, there can be a change in the relative shape, position and volume.