Question

what are the 4 steps in an ELISA test?

Answer 1

ELISA stands for enzyme linked immunosorbent assay. It is a technique that is widely used for hormone assessments, growth factors, tumor markers, bacterial or viral antigens or antibodies in biological fluids. The basic four steps in ELISA include- coating, blocking, detection and results.

• Coating : This is the very first step in ELISA . Here the well ( 96-well polystyrene plate ) is coated with the antigens or the antibodies.
• Blocking : The coating step is followed by blocking step where all the unbound sites are coated with a blocking agent(any unrelated solution of proteins).
• Detection : After blocking, the plate is now incubated with enzyme conjugated antibody or antigen which binds to a specific target antigen or antibody.
• Result : The last step includes addition of a substrate. It helps in production of calorimetric signals that produced by the substrate-enzyme complex reaction. These signals are measured and the plate is read.