Question

1. Describe the idea of color temperature?

a. What are the advantages and disadvantages of using filters to alter color temperature?

b. How does the mechanism behind a neutral density filter differ from a color correction filter? In other words, how does each of these work?

2. Sketch the four types of lens arrangements in a substage condenser and tell me what the advantages and limitations each type has.

3. How does numerical aperture relate to the resolving power of an objective?

Answer 1

1)Color temperature is a way to describe the light appearance provided by a light source . It is measured in degrees of Kelvin(K) on a scale from 1,000 to 10000K.

• For example , if you heat up a metal object , the object appears to glow . Depending on the Kelvin temperature that the metal object is being heated at, the glow will be various colours , such as orange , yellow or blue.

a) Advantages and disadvantages of using filters to alter color temperature

• Advantages is Artistic application via control of color temperature , example : vedio camera operators can white balance objects that are not white , downplay the color of the object used for white balance  
• Disadvantage is some chronic problems have been linked to exposure high energy light

b )Neutral density filter is a filter that reduces or modifies the intensity of color or wavelength.

The purpose of standard neutral-density filter is to reduce the amount of light entering the lens .

For example , one might wish to photographer a waterfall at a slow shutter speed to create a engage in long and intention of get motion-blur effect and also photography was exposed for the view through the filter and thus the remainder of the scene is over exposed ,if the exposure had instead been set for the unfiltered background, it would appear properly exposed while the view through would be dark

2) Sketch the four types of lens arrangement in a substage condenser :

Advantages and limitations

Advantages : structure can be seen clearly hence can be best used for observation of bacteria (micro organisms )

Limitations :

• n of size
• When stained , difficult to watch
• Need thinner sections with out any refractory material like oil, water droplets , air bubbles etc

3)Numerical aperture and Resolution :

​​​​​The numerical aperatur of microscope objective is a measure of its ability to gather light and resolve fine specimen detail at a fixed object distance.

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