Question

in producing a transgenic plant using the Ti plasmid of Agrobacterium tumefaciens:

a)The transgene is inserted in the Ti plasmid in place of the vir region

b)The transgene releases the tumour genes present in the T-DNA of the Ti plasmid

c)The antibiotic resistance gene on the Ti plasmid is replaced witht eh EPSP synthase gene

d) The recombinant Ti plasmids is electroporated into the plant cells

e)Recombinant plant cells are selected by growth on media containing glyphosate

Answer 1

Option B,  The transgene releases the tumour genes present in the T-DNA of the Ti plasmid

Ti plasmid also known as tumor inducing plasmid present in agro bacterium tuminificans . Ti plasmid made up of three parts mailny

--- T DNA region- contain genes which encode caixin and cytokine which promote cell growth and division. And one catabolite gene , which cleave opines and later used for carbon source. Thus T region is mainly responsible for tumor development.

--- virulence part- responsible for transfer to T gene

--- opine cstabolite gene -- responsible for catabolism of gene

In genetic engineering Ti plasmid , as this T part is not required so it is replaced with desirable gene. This desirable gene is transferred with help of virulence part Gene's.

Other option are not correct.

Vir Gene's are necessary for transfer of Gene's.

Ti plasmid is transferred with help of vector agro bacterium tuminificans.