If you examined a thioglycollate broth and it appeared light pink throughout the tube, could this...

If you examined a thioglycollate broth and it appeared light pink throughout the tube, could this tube be used for innoculation? Explain why or why not.

Expert Solution

Yes, it can be used for innoculation.

Because,

when anaerobic infection is suspected, thioglycollate medium is recommended to isolate strict anaerobes from blood.Sufficient volume of broth must be used to prevent the blood from clotting and to dilute out the blood's natural bactericidal substances. The blood should be diluted atleast 1 in 10 with broth.

Thioglycollate broth is used to find out growth characteristics of various bacteria based on their oxygen requirements.

Composition of Thioglycollate broth-

1. Cystine and casein: They supply carbon and nitrogenous compounds,

2. Dextrose: It is added as another energy source

3. Yeast extract or papaic digest of soybean meal are added as growth enhancers.

4. Sodium Thioglycollate is a reducing agent which maintains a low oxygen tension by removing molecular oxygen from the environment.

5. Sodium chloride: It maintains osmotic equilibrium.

6. Resazurin is an oxidation - reduction indicator that turns pink when increased oxidation has occurred, it is colourless when reduced.

7. Agar- The addition of small amount of agar in thioglycollate medium aids in the initiation and growth of small inocula and anaerobes by impeding the diffusion of oxygen into the medium.

genius_generous answered 3 months ago

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