In: Biology
A test tube of broth media with culture is inoculated and then incubated.
- List the four different stages of growth in the bacterial cells in the test tube
- For each of these different stages, explain what’s happens to the cell number and describe how the cells are acting metabolic.
Lag phase- No increase in the number of living bacterial cells. In this phase, considerable metabolic activity is occurring as the cells prepare to grow. This phase may not occur if the cells used to inoculate a new culture are in the log phase & provided conditions are the same.
Log phase- Exponential increase in the number of bacterial cells. Organisms in a tube of culture medium can maintain log growth for only a limited time, as nutrients are used up, metabolic wastes accumulate, microbes suffer from oxygen depletion.
Stationary phase-Plateau is observed in the graph and the growth phase is constant. the cell becomes smaller and synthesizes components to help them survive longer periods without growing, the signal to enter this phase may have to do with overcrowding
Death phase- an exponential decrease in the number of bacterial cells. Death occurs because cells have depleted intracellular ATP reserves. Not all cells necessarily die during this phase.