2) You have 25 ml of an ATP solution. You take 30 µl and dilute to...

2) You have 25 ml of an ATP solution. You take 30 µl and dilute to a final volume of 10 ml. The absorbance of this solution is 0.432. What is the concentration of the undiluted ATP solution? (molar extinction coefficient = 15,400 M-1cm-1).

If you could upload image the solution you worked out on paper, that would be preferred.

Expert Solution

2)

Absorbance = molar absorptivity x concentration x path length

assuming path length = 1 cm

we get,

concentration of diluted ATP solution = absorbance/molar absorptivity

= 0.432/15,400

= 2.80 x 10^-5 M

This is the concentration of 10 ml solution containing 30 uL (0.03 ml) of orginal solution

So,

concentration of original ATP solution = 2.8 x 10^-5 M x 10 ml/0.03 ml

= 9.33 x 10^-3 M

queen_honey_blossom answered 1 year ago

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