2) You have 25 ml of an ATP solution. You take 30 µl and dilute to...

2) You have 25 ml of an ATP solution. You take 30 µl and dilute to a final volume of 10 ml. The absorbance of this solution is 0.432. What is the concentration of the undiluted ATP solution? (molar extinction coefficient = 15,400 M-1cm-1).

If you could upload image the solution you worked out on paper, that would be preferred.

Expert Solution

2)

Absorbance = molar absorptivity x concentration x path length

assuming path length = 1 cm

we get,

concentration of diluted ATP solution = absorbance/molar absorptivity

= 0.432/15,400

= 2.80 x 10^-5 M

This is the concentration of 10 ml solution containing 30 uL (0.03 ml) of orginal solution

So,

concentration of original ATP solution = 2.8 x 10^-5 M x 10 ml/0.03 ml

= 9.33 x 10^-3 M

queen_honey_blossom answered 11 months ago

1) You have 100 ml of a solution of ATP. You take 25 ?l, and dilute...

1) You have 100 ml of a solution of ATP. You take 25 ?l, and dilute it to a final volume of 1ml. The absorbance of the diluted solution at 259 nm is 0.550. a) What is the ATP concentration in the original, undiluted solution? **The molar extinction coefficient for ATP at 259 nm is 15,600 l mol-1cm-1. b. How many mg of ATP are there in total in the original, undiluted solution? **The formula weight of ATP is 551.14...

You make two dilutions. You take 82.19 mL of the stock solution (8.1×10-5M) and dilute to...

You make two dilutions. You take 82.19 mL of the stock solution (8.1×10-5M) and dilute to 500.0 mL to make Solution #1. You then add 66.26 mL of Solution #1 to 30.11 mL of water to make Solution #2. What is the concentration of the Solution #1 in terms of molarity? What is the concentration of the Solution #2 in terms of molarity?

you have 1-5 ml of 0.140 M HCl solution and you want to dilute it to...

you have 1-5 ml of 0.140 M HCl solution and you want to dilute it to exactly 0.100 M. How much water should you add?

To what volume (in mL) should you dilute 100.0 mL of a 5.00 M CaC2 solution...

To what volume (in mL) should you dilute 100.0 mL of a 5.00 M CaC2 solution to obtain a 0.750 M CaC2 solution?

To what volume (in milliliters) should you dilute 100.0 mL of a 4.50 M solution of...

To what volume (in milliliters) should you dilute 100.0 mL of a 4.50 M solution of CaCl2 solution to obtain a 0.900 M solution of CaCl2? Express the volume to three significant figures and include the appropriate units.

To what volume should you dilute 133 mL of an 7.95 M CuCl2 solution so that...

To what volume should you dilute 133 mL of an 7.95 M CuCl2 solution so that 49.0 mL of the diluted solution contains 4.27 g CuCl2? Express your answer in milliliters.

Your supervisor wants you to dilute a 1 mL stock BSA solution (1 mg/mL) into 1:10...

Your supervisor wants you to dilute a 1 mL stock BSA solution (1 mg/mL) into 1:10 dilutions. What is the final concentration of diluted BSA solution? Show your work. How much volume (mL) of BSA and distilled water would you need to perform the dilution? Show your work.

Concentrated hydrochloric acid solution is 37.0% HCL and has a density of 1.19g/ml. A dilute solution...

Concentrated hydrochloric acid solution is 37.0% HCL and has a density of 1.19g/ml. A dilute solution of HCL is prepared by diluting 4.50mL of this acid to 100.00mL with water. Then 10.0mL of the dilute HCL is used for the reaction with AgNO3 solution as shown below: HCL(aq)+AgNO3(aq) to HNO3(aq)+AgCL(s) How many mL of 0.1105M AgNO3 solution is required to precipitate all of the chloride as AgCl(s)? Please show how you get the answer. Thank you!

Suppose you have 300 ml of an aquesous solution that contains 30 g of malononitrile. To...

Suppose you have 300 ml of an aquesous solution that contains 30 g of malononitrile. To isolate malononitrile from this aqueous solution you decide to use ether as the organic solvent in your liquid-liquid extraction. The solubility of malononitrile in either at room temperature is 20.0g/ 100mL and in water is 13.3g/100mL. Consider the following: a. How many grams of malononitrile can be isolated with three extractions using 100 mL of ether each time? b. How many grams of malononitrile...

A dilute aqueous solution of sulfuric acid at 25°C is used to absorb ammonia in a...

A dilute aqueous solution of sulfuric acid at 25°C is used to absorb ammonia in a continuous reactor, thereby producing ammonium sulfate, a fertilizer: 2NH3(g) + H2SO4(aq) ? (NH4)2SO4(aq) a)If the ammonia enters the absorber at 75°C. the sulfuric acid enters at 25°C, and the product solution emerges at 25°C, how much heat must be withdrawn from the unit per mol of (NH4)2SO4 produced? b)Estimate the final temperature if the reactor of part (a) is adiabatic and the product of...

Question