What is homologous recombination? What is site-directed recombination?

Expert Solution

Homologous recombination is a type of genetic recombination in which nucleotide sequences are exchanged between two similar or identical molecules of DNA. It is used by cells to correctly repair harmful breaks, which take place on both strands of DNA. This recombination produces new combinations of DNA sequences during the process of meiosis. These new combinations of DNA characterize genetic variation in offspring, which allows populations to adapt during the evolution. It is used in horizontal gene transfer to exchange genetic material between different strains and species of bacteria and viruses.

Site-directed recombination is a type of genetic recombination in which DNA strand exchange occurs between segments possessing at least a certain degree of sequence homology. It perform rearrangements of DNA segments by recognizing and binding to short DNA sequences at which they slice the DNA backbone, exchange the two DNA helices involved and rejoin the DNA strands. They are highly specific, fast and efficient. They are utilized in a variety of cellular processes, which include bacterial genome replication, differentiation and pathogenesis and movement of mobile genetic elements.

gladiator answered 2 years ago

What is homologous recombination? What is site-directed recombination?Compare and contrast the two processes. Address their principles,...

What is homologous recombination? What is site-directed recombination?Compare and contrast the two processes. Address their principles, molecular mechanisms, and physiological functions.

1. What is homologous recombination? What is site-directed recombination? Compare and contrast the two processes. Address...

1. What is homologous recombination? What is site-directed recombination? Compare and contrast the two processes. Address their principles, molecular mechanisms, and physiological functions.

Compare and contrast homologous recombination and site specific recombination. Address their principles, molecular mechanisms, and physiological...

Compare and contrast homologous recombination and site specific recombination. Address their principles, molecular mechanisms, and physiological functions

Explain what is meant by the term ‘linked genes’ with respect to homologous recombination. For example,...

Explain what is meant by the term ‘linked genes’ with respect to homologous recombination. For example, if an organism is heterozygous at two genetic loci on the same chromosome, in what way does the position of these alleles with respect to each other affect how they are transmitted to gametes during meiosis? You have read that chromosomes carry the basic genetic information required for all of our cells. Chromosomal abnormalities are the cause of various health conditions and can occur...

Non-homologous end joining (NHEJ) and Homologous recombination repair (HRR) are important DNA repair mechanisms in the...

Non-homologous end joining (NHEJ) and Homologous recombination repair (HRR) are important DNA repair mechanisms in the mammals. With diagrams, explain how the repairs are carried out in NHEJ and HRR. Are they completely independent pathways? Justify your answer with specific examples.

1) What are double-strand breaks, and how does homologous recombination repair them? 2) What types of...

1) What are double-strand breaks, and how does homologous recombination repair them? 2) What types of genes are transcribed by RNA polymerase I, II, III? Be sure that it is clear which genes are for which polymerase. Please only answer in sentences no pictures, etc.

Please describe site-directed mutagenesis.

DNA shuffling is a method for in vitro homologous recombination of pools of selected mutant genes...

DNA shuffling is a method for in vitro homologous recombination of pools of selected mutant genes by random fragmentation and polymerase chain reaction (PCR) reassembly. In our class, we went through a paper describing the evolution of four cephalosporinase from various species. The paper showed that a single cycle of shuffling yielded 8-fold improvements from the four separately evolved genes, versus a 270- to 540-fold improvement from the four genes shuffled together (so called family shuffling). Discuss how this result...

components for conservative site-specific recombination involving Cre recombinase?

1. Homologous recombination in meiosis has the main purpose of_____ a. Dna repair b. creating new...

1. Homologous recombination in meiosis has the main purpose of_____ a. Dna repair b. creating new chromosomes c.sealing double stranded breaks - i think the answer is creating new chromosomes since it lines up in pairs and swaps segments of dna 2. The invading end is usually a _______ a. 3' end b. 5' end c. both - I think it is 3' end because I couldnt find 5' end in text book 3. The extension of the paired region...

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