-Describe the improvment that led to dye-terminator sequencing. -Determine the DNA sequence based on hypothetical data...

-Describe the improvment that led to dye-terminator sequencing.

-Determine the DNA sequence based on hypothetical data derived from sanger and dye-terminator sequencing.

what about the first question

Expert Solution

The basic Sanger sequencing method is a DNA sequencing done by the incorporation of selective chain-terminating ddNTPs by DNA polymerase. 4 reactions are needed to set for 4 different ddNTPs. After the DNA extension, the samples are run on the gel and observe under UV for sequencing.

But in Automated, high throughput DNA sequencing, the dye-labeled primers and ddNTPs are used for better sequencing. In dye-terminator sequencing only the terminator ddNTPs are dye-labeled.

The dye-terminator sequencing is very convenient as it requires only a single reaction and there is no need for labeled primer also. Here only the sequence of DNA having labeled terminator is detected. The dye-terminator sequencing method, along with automated high-throughput DNA sequence analyzers, was used for the vast majority of sequencing projects until the introduction of Next Generation Sequencing.

gladiator answered 6 months ago

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