Question

In: Biology

1. In eukaryotic mRNA splicing, which snRNP binds to the branch point A? A. U1 B....

1. In eukaryotic mRNA splicing, which snRNP binds to the branch point A?

A. U1

B. U2

C. U3

D. U4

E. U5

F. U6

2. Transcription of protein coding genes in prokaryotes and eukaryotes differs in which way?

A. The direction of synthesis of the RNA polynucleotide

B. The requirement for a primer

C. The requirement for the promoter sequence

D. The requirement for a promoter sequence

E. The requirement for excision of introns

3. During transcription from a basal promoter in prokaryotes, which of the following is/are true?

A. The replication fork sets out in both directions

B. Sigma factor is required for correct start site selection

C. Termination requires nonsense condons

D. Termination uses rho factor or hairpin structures

Expert Solution

Answer 1- There are 5 snRNPs U1,U2,U4,U5,U6 associated in mRNA splicing. U1 binds to 5' splice site, U2 binds to branch point, it forms the prespliceosomal complex it brings the 5'splice site in close proximity to branch point.

Spliceosome is formed when U4/U6 snRNP and U5 snRNP binds to prespliceosomal complex, and spliceosomal complex leads to splicing process

Hence option B U2 is correct.

Answer-2 Transcription in prokaryotes is generally combined with translation, i.e as before completion of transcription, translation starts. introns are not present in prokaryotic mRNA so splicing process doesnot take place.

But eukaryotic mRNA consists of exons and introns both so before translation removal of introns is done by the process known as splicing.

Hence the difference in transcription in prokaryotes and eukaryoes is that in eukaryotes splicing of introns takes place.

Hence option E is correct.

Answer- 3 Transcription is the process in which mRNA is formed, in transcription one DNA strand acts as template and one as coding strand. Transcription is a unidirection process in which mRNA is formed in 5' to 3' direction.

Transcription starts by binding of sigma factor which recognizes the promoter sequence and facilitates binding of core polymerase which carry out elongation process, elongation takes place after removal of sigma factor, after elongation termination takes place termination is of two types it may be rho dependent in which rho factor is required for termination or it may be rho independent in which termination stops after reaching non sense or stop codons.

Hence here option B,C,D are true.

gladiator answered 2 years ago

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