You think you have a receptor with three identical and independent ligand binding sites that came...

You think you have a receptor with three identical and independent ligand binding sites that came about from trimerization of monomeric receptors. If you knew what the signal was for binding to one monomer, would a binding titration be able to prove that the trimer is being formed? What are some techniques to prove it exists as a trimer in solution (i.e. that its molecular weight is three times the monomer)?

Expert Solution

queen_honey_blossom answered 8 months ago

Think of a toy model of a dimeric receptor binding with a divalent ligand. The KDs...

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