Suppose an enzyme has Km = 0.1mM and kcat = 10^3 s-1, and you
perform an experiment in which [E]tot = 10-7M. Suppose that adding
0.1 µM of an inhibitor results in a Kmeff= 0.5 mM, and has no
effect on kcat. Determine the substrate concentation that would be
necessary to achieve a reaction velocity v of 5*10^-5Ms-1
The data for an Enzyme with a Kcat is 5,000m-1. This experiment
was performed by adding a given amount of substrate of 100
micrograms of enzyme in buffer solution. What is the molecular
weight of the enzyme?
Substrate Concentration mM
rate (Vo) micromol/min
1
167
2
250
4
334
6
376
100
498
1000
499
If a solution contains 7.5 X 10-8M Cu+2
and 1.0 X 10-7 M Zn+2 will either metal
precipitate as a hydroxide salt if the pH is 8.5? At what
concentration of OH- will each metal precipitate?
1.For the diprotic weak acid H2A, Ka1 = 2.5 × 10-6 and Ka2 = 7.5
× 10-9. What is the pH of a 0.0400 M solution of H2A? What are the
equilibrium concentrations of H2A and A2– in this solution?
2.HClO is a weak acid (Ka = 4.0 × 10–8) and so the salt NaClO
acts as a weak base. What is the pH of a solution that is 0.026 M
in NaClO at 25 °C?
3.NH3 is a weak...
10. Continuing with the same population in Question 7, let us
consider the case where we picked a sample of size 14. (Do not
worry
about the fact that it is stupid to have a sample of size 14
when the population has only 4 people in it.) What is the standard
error
(deviation) of the sampling distribution of the mean?
11. You need to clean out a fish tank, so you get a small bowl
to take out all...
Run No.
Cup
Temp. (˚C)
Time (sec.)
1
C
10
139
1
RT
22
48
1
H
46
21
2
C
6
162
2
RT
22
45
2
H
44
20
3
C
8
149
3
RT
22
48
3
H
42
20
Avg.
C
8
150
Avg.
RT
22
47
Avg.
H
44
20.33
Using graphing software such as Excel®, graph the
results of Activity 1. Please see the Introduction to Graphing
manual for guidance.
a. What are...
A=
1
0
-7
7
0
1
0
0
2
-2
10
-7
2
-2
2
1
Diagonalize the matrix above. That is, find matrix D and a
nonsingular matrix P such that A = PDP-1 . Use the
representation to find the entries of An as a function
of n.
The decomposition of N2O5 occurs with a rate constant of 4.3 x
10–3 sec–1 at 65C and 3.0 x 10–5 sec–1 at 25C. What is the
activation energy of the process?
Vadercat Limited issued $50 million 7.5 percent, 10 year bonds
on October 1, 2019. The market rate of interest on the date of the
issue was 8 percent. Interest is payable semi-annually on April 1
and October 1. The company’s year-end is December 31.
Required:
a. Prepare journal entries to record all transactions during the
first year the bonds are outstanding. The company uses the
straight-line method of amortizations.
b. Indicate how the bond obligation would be shown on the...