Question

1. Incubation conditions can influence what organisms grow. What type of conditions would you want to grow your sample in? Why?

2. Use Google or your other favorite search method and find the ingredients and method for making each of the plates listed in the introduction. Type this information out yourself here and don’t forget to reference where you found the information! (Tryptic Soy Agar, Tryptic Soy Agar supplement with 0.5M NaCl, R2A supplement with 1g/L tryptone and 0.5M NaCl, Margin Agar)

Answer 1

For othwr organisms used in biological experiments, such as the budding yeast Saccharomyces csrevisiae, a growth temperature of 30°C (86°F) is optimal. More elaborate incubators can also include the baility to lower the temperature (via refrigeration), or the ability to control humidity or CO​​​​​2 levels.

Making Agar plates- Agar plates are the standard solid support material for growing microorganisms. These are energy rich, nutrient providing and fuel the microbes as they grow..

On solid media, a single microbe will grow and divide to produce a colony, a spot of identical descendants.

Tryptic soy agar contains digests of casein and soybean meal. The combination of casein and soy peptones renders the medium nutritious by supplying organic nitrogen, particularly and amino acids and longer chines peptides.

Tryptic soy agar supplements- Pancreatic digest of soybean meal (10.0 gm), peptic digest of soybean meal (0.5 gm), Sodium chloride (0.5 gm), agar (10.0 gm).

R2A supplement- Dipotassium phosphate (0.3g/L), Magnesium sulfate heptahydrate (0.05g/L), Sodium pyruvate (0 .3g/L), Agar (15.0g/L)