Question

what is the sensitivity off CCD & Wavenumber in Raman spectroscopy and how it affects the intensity of the peak??

Answer 1

What limits the CCD performance?
1. Read noise: How many photon generated electrons are required to achieve
a signal level greater than the read noise?
Raman systems that require off chip binning increase read noise to the
square root of the number of pixels binned.
2. Dark Charge rate: How long can you integrate before the binned CCD
pixels generate a charge equivalent to the read noise?
At the integration time that the dark charge signal contributes to the noise
either through shot noise or uniformity of response, it must be subtracted.
3. Uniformity of response: How many photon generated electrons can be
measured before the shot noise is exceeded by the non-uniformity of
response?
At the point uniformity of response noise exceeds shot noise the pixels must
be read out individually (without binning) for response correction.