Which spectroscopy is more sensitive: fluorescence or
absorption? Why? (2) Between fluorescence and phosphorescence:
which one has a higher intensity? Which one lasts longer?
Describe at least one analytical technique that you could use
that is more sensitive than Raman spectroscopy, FTIR spectroscopy,
and energy-dispersive X-ray spectroscopy (EDS) analyses, but that
might be destructive in its analysis of the material. Describe the
benefits and other limitations of this technique.
a) Consider three common types of phonon spectroscopy, Raman
spectroscopy, Brillouin spectroscopy and inelastic neutron
scattering. Describe the differences between the three techniques
in terms of the following variables and comment briefly on the
limitations of each technique as a consequence of these
differences.
i) The phonon wavevector range probed by each technique.
ii) The range of energy transfers to be probed by each
technique.
iii) The momentum (wavenumber) resolution of each technique.
1. Why are the thermal detectors slower and less sensitive than
the photon detectors?
2. What are the physical origins of the shot noise and Johnson
noise in an optical detector?
Why is Raman spectroscopy so useful for on-line process analysis
or in-situ measurements?
discuss the importance or reasons for a traceable relative
intensity correction in Raman spec.
what advantages does Raman have over IR or UV-Vis?
why does the measurement of fluorescence data require
a more intense source of uv/visible spectroscopy? specific. b) what
is the typical source for spectofluorometer? c) for the
photomuiltiplier transducer what is the mode off operation used
signal to noise?
Describe two major reasons why fluorescence spectrophotometry is
a very sensitive analytical technique? (hint, one of the reasons
involves the equation I = KP0C)
If you wish to analyze a molecule using fluorescence
spectrophotometry but that molecule does not fluoresce, what can
you do?
What characteristics of a molecule make it likely to
fluoresce?