In: Biology
A purple flowering, smooth seed dihybrid plant (genotype PpSs) is test crossed with a white flowering, wrinkled seed plant (genotype ppss). These produce progeny in the following numbers of four phenotypes: 24:76:74:26 (purple flower + smooth seed coat: purple flower + wrinkled seed coat: white flower + smooth seed coat: white flower + wrinkled seed coat). a) Explain how ratios of progeny show that the two genes are linked. b) How many map units separate the colour and seed coat genes? Show your calculations.
Answer: The genotype of Purple flowering smooth seed dihybrid plant is PpSs.
Test crossed with white flowering wrinkled seed plant (genotype ppss)
Purple flowering smooth seed X white flowering wrinkled seed
PpSs X ppss
gametes PS, Ps, pS, ps X ps
PS | Ps | pS | ps | |
ps |
PpSs Purple smooth |
Ppss Purple wrinkled |
ppSs white smooth |
ppss white wrinkled |
a) The following numbers of progenies are obtained from the above test cross.
24:76:74:26
24 purple flower + smooth seed coat: 76 purple flower + wrinkled seed coat: 74 white flower + smooth seed coat: 26 white flower + wrinkled seed coat. This ratio indicates that the genes responsible for flower color and sead coat are linked.
b) To find the map units that separate the colour and seed coat genes, we have to calculate the recombinant frequency between the genes P and S.
The total number of progenies = 200
So the recombinant frequency between the genes P and S = 76 + 74 / 200 = 150 / 200 = 0.75 x 100 = 75 map units
The map units that separate the colour and seed coat genes = 75 map units.