In: Chemistry
An HEXAPEPTIDE was sequenced using differing enzyme and chemical reagents. The results of these experiments, along with an explanation of how to interpret the results are provided below. Using this information, provide the 1 ̊ structure for this HEXAPEPTIDE.
Show work/answer for points B-F
Observations from Sequencing Experiments
A. The amino acid content in the peptide was: Y, R, M, K, G, D
B. Reactions with the peptide and 1-fluoro-2,4-dinitrobenzene yielded DNP-G.
C. Treatment of the peptide with chymotrypsin yielded two tripeptides.
D. Treatment of the peptide with trypsin yielded three dipeptides.
E. Treatment of the peptide with carboxypeptidase yielded methionine.
F. N-terminal sequencing of one the chymotrypsin-produced tripeptides (from part C) yielded DNP-K.