Question

Fill in the blanks:

1.What growth plating medium would selectively isolate Staphyloccus aureus from an incriminated food in staph poisoning ____________ and what would be gram reaction/morphology/grouping________ and the main symptom(s) of staph food poisoning __________and timing (choose hours or days) of appearance of symptoms if positive and consumed?__________

2. What growth plating medium would selectively isolate Vibrio cholerae from an infected patient ________ and what would be Gram staining reaction/morphology/grouping____________and the main symptom(s) ____________ and timing (Choose hours or days) of apearance of the symptoms if positive and consumed?_________

Answer 1

Solutions

1.

What growth plating medium would selectively isolate Staphyloccus aureus from an incriminated food in staph poisoning	The growth medium that would be chose is Mannitol salt agar. It is a commonly used selective and differential growth medium. The presence of mannitol is used as a differentiating agent, since s.aureus colonies produce yellow colonies. The high salt concentration inhibits growth of most organisms.
what would be gram reaction/morphology/grouping	Gram-positive, round-shaped bacterium that is a member of the Firmicutes.
the main symptom(s) of staph food poisoning	Nausea, explosive vomiting, abdominal cramps/pain, headache, weakness, and diarrhea
timing of appearance of symptoms if positive and consumed	1 – 6 hours for rapid onset of symptoms.

2.

What growth plating medium would selectively isolate Vibrio cholera from an infected patient	Thiosulfate-citrate-bile salts-sucrose agar, or TCBS agar, is highly selective towards the isolation of Vibrio species. The presence of bile salts and alkaline pH inhibits the growth of most bacteria, except Vibrio, which ferments the saccarose sugars to produce yellow colonies.
what would be gram reaction/morphology/grouping	Gram-negative, comma-shaped bacterium
the main symptom(s)	Watery diarrhea, Abdominal cramps, Nausea, Vomiting, Fever, Headache.
timing of appearance of symptoms if positive and consumed	Between 12 hours and 5 days after exposure