Biotechnology of microorganisms. You wish to synthesize an antibiotic (a protein) using a bacterium which does...

Biotechnology of microorganisms.
You wish to synthesize an antibiotic (a protein) using a bacterium which does not have that gene.
(a) Draw a diagram of the plasmid you would use to transform the bacteria.
(b) How would you select the bacteria containgin the plasmid?
(c) Do you think it would be appropriate to use a species which allow a high number of copies of the same plasmid? Why?
(d) Do you think an inducible promoter is needed to control the expression of the antibiotic gen? Why?
(e) Do you think we should include a suicide gene in the vector? Why?
(f) Do you think it would be beneficial if the bacteria secreted the antibiotic to the medium? Why?
(g) Do you think it is necessary for the culture in the reactor to be axenic?

Expert Solution

gladiator answered 2 years ago

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Biotechnology of microorganisms. You wish to synthesize an antibiotic (a protein) using a bacterium which does...

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