Question

1. You want to express Erythropoietin (EPO) in mass quantities. EPO is a glycoprotein hormone responsible for the control of RBC synthesis in the bone marrow. It has invaluable medical usages in animals and cancer therapy. It is important to note that it has highly complex glycosylations.

1. Which expression system would you use to appropriately and efficiently express your gene of interest? Briefly explain the reason for your choice.

1. b. You implement your gene of interest into your designed expression system. However, after your extraction step, you are not able to obtain the desired protein of interest. List one possible reason why this occurred and state how you would address this problem.
2. c. You decide to produce your desired protein in large batches. You obtain a small sample from your bioreactor and observe “clumps” within the cells. What do you think those could be? Based on this answer, propose a possible solution.

Answer 1

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