Question 4(BOTANY) You want to clone and express a gene that you've isolated from Harpagophytum in...

Question 4(BOTANY)

You want to clone and express a gene that you've isolated from Harpagophytum in E.coli describe all the steps that you would take to clone your gene into E. coli. Draw a diagram of your vector containing the gene insert. Discuss how you will identify a clone in an E. coli cell that you can work further with. Describe the steps that you will follow in detail and provide details of the techniques that you use in each step and how your approach will impact on these steps. (25)

Expert Solution

gladiator answered 2 years ago

Question 3(BOTANY) You want to clone and express the gene that you’ve isolated from devil’s claw...

Question 3(BOTANY) You want to clone and express the gene that you’ve isolated from devil’s claw in E. coli. Describe all the necessary adjustments that you would need to make to your gene to ensure correct expression in E. coli. (5)

The human gene for a protein hormone has been isolated. Scientists want to introduce this gene...

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1) You wish to clone a gene into a bacterial plasmid for the production of the...

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4. You want to clone a 1.5 kb cDNA. Which vectors discussed would be appropriate to...

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How would you clone the Eukaryotic gene into the plasmid How would you transform bacteria with...

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Describe, in detail, how you would obtain a gene from a tomato plant and express this...

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3) How can you use PCR to clone a 2000bp gene into a plasmid vector?

You decide to clone a gene using a plasmid vector featuring ampicillin resistance and the lacZ...

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Due to system limitations you are attempting to clone a small bacterial gene using a poorly...

Due to system limitations you are attempting to clone a small bacterial gene using a poorly characterised bacterial plasmid. You know that the plasmid contains only two drug resistance markers (one for resistance to tetracycline, another for resistance to ampicillin). You have determined that the restriction enzymes EcoRI and BamHI each cut the plasmid only once while SauIII and RsaI cut the plasmid twice. You also have determined that the EcoRI and SauIII recognition sites are within the tetracycline resistance...

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