Question 4(BOTANY) You want to clone and express a gene that you've isolated from Harpagophytum in...

Question 4(BOTANY)

You want to clone and express a gene that you've isolated from Harpagophytum in E.coli describe all the steps that you would take to clone your gene into E. coli. Draw a diagram of your vector containing the gene insert. Discuss how you will identify a clone in an E. coli cell that you can work further with. Describe the steps that you will follow in detail and provide details of the techniques that you use in each step and how your approach will impact on these steps. (25)

Expert Solution

gladiator answered 2 years ago

Question 3(BOTANY) You want to clone and express the gene that you’ve isolated from devil’s claw...

Question 3(BOTANY) You want to clone and express the gene that you’ve isolated from devil’s claw in E. coli. Describe all the necessary adjustments that you would need to make to your gene to ensure correct expression in E. coli. (5)

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