The protein encoded by CDC33 has a molecular weight of 24 kD. There are about 6...

The protein encoded by CDC33 has a molecular weight of 24 kD. There are about 6 picograms of TOTAL protein per haploid yeast cell. The Cdc33 protein is present in 3000 copies per cell. What fraction of the total protein in a yeast cell is Cdc33 protein?

Expert Solution

mass of the CDC33 protein= 24 kD

1 kDa=10^3 Da

so 24 kDa= 2410^3 Da

1Da= 1.66053 10^-24 g

so the mass of 3000 copies of CDC33 protein= 30002410^3 1.66053 10^-24= 1.1210^-16 g

1 g= 10^12 pg

so 1.1210^-16 g= 1.1210^-16 10^12=1.1210^-4 pg

mass of total protein in the yeast cell = 6 pg

fraction of CDC33 protein out of the total protein in a yeast cell = total mass of CDC33/total mass of protein=1.1210^-4 pg/6pg= 210^-5

gladiator answered 9 months ago

Protein X has a Kd of 0.25 micromolar; protein Y has a Kd of 0.5 micromolar,...

Protein X has a Kd of 0.25 micromolar; protein Y has a Kd of 0.5 micromolar, and protein Z has a Kd of 0.75 micromolar for ligand A. Which one of the following is true? Group of answer choices 1-Protein X has the highest affinity for ligand A. 2-Protein Z has the highest affinity for ligand A. 3-Protein X has the lowest affinity for ligand A. 4-Protein Y affinity for ligand A is higher than that of protein X.

A certain purified protein has a molecular mass expressed as x kD (1 D = 1/12...

A certain purified protein has a molecular mass expressed as x kD (1 D = 1/12 x mass of 12C atom). Show that an aqueous solution of this protein expressed as x mg/mL is equivalent to a protein concentration of 1mM

You are given a sample that has 1.3 mg/ml of purified protein. The molecular weight is...

You are given a sample that has 1.3 mg/ml of purified protein. The molecular weight is 37,000 Daltons. a) the absorbance in a 1cm cuvette is 0.93. calculate the molar extinction coefficient. Be sure to express your answer with correct units. b)also calculate the extinction coefficient for concentration units of mg/ml, i.e. with units of cm-1 (mg/ml)-1 c) The sample is actually contaminated with 0.01 mg/ml DNA, but the protein concentration, 1.3 mg/ml is correct. explain how the contaminating DNA...

An antifreeze protein, with a molecular weight of 17000 Da, was found to be present at...

An antifreeze protein, with a molecular weight of 17000 Da, was found to be present at a concentration of 10g dm-3 in the serum of an Antarctic fish. calculate the expected depression of the freezing point and compare your result with 1) the observed depression of 0.6K and 2) the depression of 0.0014K observed for 10g dm-3 solution of lysozyme, molecular weight of 14500 Da. for pure water, Lf=6.02kj ml-1 and Tm=273.15 K

WHAT DOES THE ENCODED PROTEIN DO IN THE BODY?

You have discovered a new transmembrane protein that resides in the ER. The molecular weight of...

You have discovered a new transmembrane protein that resides in the ER. The molecular weight of the protein is approximately 100 Kd and it contains a hydrophobic transmembrane domain in middle of the protein amino acid sequence. You want to determine whether the protein is a Type I or Type II transmembrane protein. For the experiment, you are given the following reagents: cDNA construct for the protein, a cell line, a centrifuge, SDS-page apparatus, reagents needed for Western blot analysis...

Protein Molecular Weight Isoelectric point Does the protein contain heme moiety? 1 68,000 5.5 No 2...

Protein Molecular Weight Isoelectric point Does the protein contain heme moiety? 1 68,000 5.5 No 2 15,000 5.0 No 3 17,500 9.5 Yes Could gel filtration chromatography be used to separate a mixture containing proteins 1 & 3? If gel filtration chromatography can be used, which protein would elute las? After collection fractions from the column, the absorbance of each fraction will be measured in a spectrophotometer - can both proteins 1 & 3 be monitored at 280 nm and...

Separation of protein by molecular weight by adding negative charge to them is: 1. electricity 2....

Separation of protein by molecular weight by adding negative charge to them is: 1. electricity 2. SDS 3. flow from cathode to anode 4. adjusted pH 5. Dialysis 5.

6) A solution of sodium chloride (molecular weight 58.5) is electrolyzed and it is found that...

6) A solution of sodium chloride (molecular weight 58.5) is electrolyzed and it is found that a current of 1 A liberates 1.3 x 10-3 kg of chlorine (atomic weight 35.5) in one hour. Sodium chloride crystals of density 2.17 x 103 kg/m3 are analyzed by x rays and the unit cell parameter is found to be 5.6 x 10-10 m. From these data calculate the charge on a monovalent ion. 7) In a Milikan oil-drop experiment, a certain droplet...

You have a mixture of the proteins listed below. Protein pI Molecular Weight (kDa) A 3.1...

You have a mixture of the proteins listed below. Protein pI Molecular Weight (kDa) A 3.1 265 B 6.9 93 C 10.3 96 D 7.1 43 E 8.6 189 1) You load the protein mix onto a cation exchange column at pH 5. Next, you apply a "washing" step by passing through buffer at pH 5. Finally, for your elution step, you apply a pH gradient starting from pH 2.0 to pH 13.0. (A gradient buffer system allows you to...

Question