In: Biology
The protein encoded by CDC33 has a molecular weight of 24 kD. There are about 6 picograms of TOTAL protein per haploid yeast cell. The Cdc33 protein is present in 3000 copies per cell. What fraction of the total protein in a yeast cell is Cdc33 protein?
mass of the CDC33 protein= 24 kD
1 kDa=10^3 Da
so the mass of 3000 copies of CDC33 protein= 300024
10^3
1.66053
10^-24= 1.12
10^-16
g
1 g= 10^12 pg
so 1.1210^-16
g= 1.12
10^-16
10^12=1.12
10^-4
pg
mass of total protein in the yeast cell = 6 pg
fraction of CDC33 protein out of the total protein in a yeast
cell = total mass of CDC33/total mass of protein=1.1210^-4
pg/6pg= 2
10^-5