A certain purified protein has a molecular mass expressed as x kD (1 D = 1/12...

A certain purified protein has a molecular mass expressed as x kD (1 D = 1/12 x mass of 12C atom). Show that an aqueous solution of this protein expressed as x mg/mL is equivalent to a protein concentration of 1mM

Solutions

Expert Solution

1 mL has x mg

V = 1 mL = 1*10^-3 L
mass = x mg = x*10^-3 g
molar mass = x KD = x*10^3*(1/12)*12 g = x*10^3 g
number of moles = mass / molar mass
=x*10^-3 / (x*10^3)
= 10^-6 mol

Concentration = number of moles / volume
                               = 10^-6 mol / 10^-3 L
                               =10^-3 mol/L
                              = 10^-3 M
                               = 1 mM

Proved

queen_honey_blossom answered 1 year ago

Next > < Previous

Related Solutions

Protein X has a Kd of 0.25 micromolar; protein Y has a Kd of 0.5 micromolar,...

Protein X has a Kd of 0.25 micromolar; protein Y has a Kd of 0.5 micromolar, and protein Z has a Kd of 0.75 micromolar for ligand A. Which one of the following is true? Group of answer choices 1-Protein X has the highest affinity for ligand A. 2-Protein Z has the highest affinity for ligand A. 3-Protein X has the lowest affinity for ligand A. 4-Protein Y affinity for ligand A is higher than that of protein X.

The protein encoded by CDC33 has a molecular weight of 24 kD. There are about 6...

The protein encoded by CDC33 has a molecular weight of 24 kD. There are about 6 picograms of TOTAL protein per haploid yeast cell. The Cdc33 protein is present in 3000 copies per cell. What fraction of the total protein in a yeast cell is Cdc33 protein?

You are given a sample that has 1.3 mg/ml of purified protein. The molecular weight is...

You are given a sample that has 1.3 mg/ml of purified protein. The molecular weight is 37,000 Daltons. a) the absorbance in a 1cm cuvette is 0.93. calculate the molar extinction coefficient. Be sure to express your answer with correct units. b)also calculate the extinction coefficient for concentration units of mg/ml, i.e. with units of cm-1 (mg/ml)-1 c) The sample is actually contaminated with 0.01 mg/ml DNA, but the protein concentration, 1.3 mg/ml is correct. explain how the contaminating DNA...

A protein is purified using ultracentrifugation and found to have a mass of 300 kDa. It...

A protein is purified using ultracentrifugation and found to have a mass of 300 kDa. It is further separated on SDS-PAGE and is found to have a single band corresponding to a mass of 100 kDa. When the protein is treated with beta-mercaptoethanol (breaks disulfide bonds), the SDS-PAGE shows 2 bands, one at 20 kDa and one at 80kDa. Describe the tertiary and quaternary structure, include forces holding the protein together (Hint use prefixes like homo- or dodeca-) So far...

A nurse identifies 12 mm of induration at the site of a tuberculin purified protein derivative...

A nurse identifies 12 mm of induration at the site of a tuberculin purified protein derivative (PPD) test when a returns to the health office to have it readWhat does the nurse explain to the client about this testi

A protein was purified to homogeneity. Determination of the mass by gel-filtration chromatography yields 60 kDa....

A protein was purified to homogeneity. Determination of the mass by gel-filtration chromatography yields 60 kDa. Chromatography in the presence of 6 M urea yields a 30-kDa species. When the chromatography is repeated in the presence of 6 M urea and 10 mM β-mercaptoethanol, a single molecular species of 15 kDa results. Which of the following best describes the structure of the protein? A. a dimer of 30 kDa subunits linked by disulfide bonds B. a tetramer of 15 kDa...

1. Calculate the molecular mass (in g/mole) for heavy water, D2O, where D is 2H. 2....

1. Calculate the molecular mass (in g/mole) for heavy water, D2O, where D is 2H. 2. Calculate based on the relative abundance of the three main isotopes of silicon the average atomic mass of Si and compare your results with the value in your Table of Elements (28Si(92.2%), 29Si(4.7%), (30Si(3.1%)) 3. Calculate the molar mass of H2SO4 , KClO4, and H3PO4. 4. How many grams of deuterium (D is 2H) are in 50 grams of Heavy water (D2O)? 5. A...

Describe one advantage and one disadvantage of trying to solve molecular protein structures by 1) X-ray...

Describe one advantage and one disadvantage of trying to solve molecular protein structures by 1) X-ray crystallography and by 2) Nuclear Magnetic Resonance (NMR) spectroscopy

1) Two identical blocks of mass M slide on opposite frictionless tracks obeying y(x) = D((e^-x/D)-1)...

1) Two identical blocks of mass M slide on opposite frictionless tracks obeying y(x) = D((e^-x/D)-1) and y(x) = D((e^x/D)-1) repectively. There is gravity g. A spring of equilibrium length zero and spring constant k is stretched between the masses, initially separated by 2D. Both blocks are released from rest simultaneously and begin to slide down their planes, reducing the stretch of the spring between them. a) Find the maximum value of k that will keep the blocks from leaving...

A mammalian cell nucleus contains 4.0 x 10 ^ 3 molecules of protein X (molecular weight...

A mammalian cell nucleus contains 4.0 x 10 ^ 3 molecules of protein X (molecular weight 6.0 x 10 ^4). Assume the nucleus is a sphere with diameter of 5.0 microns. Calculate the following: A) the molarity of protein X in the nucleus B) the mass (in grams) of protein X in one nucleus. Please show work.

Subjects