In: Chemistry
0.5 microlitres of serum is added in tube 1, which contains 1.5 microlitres of diluent .I have 4 more tubes with 1.0 microlitres of diluent .I do a serial dilution using 0.5 microlitres from the previous tube and adding it to next .What is the dilution factor of tube 1 ?what is dilution factor of tube 5?If i tell you the serum value was 648mg in pure form .How many mg should be in Tube 5?Now the other way ,if tube 5 has 2mg of substance what will you report to physician?