In: Biology
Discuss with examples the genetic mechanisms that deregulate the cell cycle in tumors.(Min 2 and a half pages)
The ultimate outcome of all growth-promoting stimuli
is the entry of quiescent cells into the cell cycle. Cancers may
become autonomous if the genes that drive the cell cycle become
dysregulated by mutations or amplification. Before further
consideration of this aspect of carcinogenesis, a brief review of
the normal cell cycle is warranted.
The Normal Cell
Cycle
Cell proliferation is a tightly controlled process that
involves a large number of molecules and interrelated
pathways. The replication of cells is stimulated by growth factors
or by signaling from ECM components through integrins. To achieve
DNA replication and division, the cell goes through a tightly
controlled sequence of events known as the cell cycle. The cell
cycle consists of G1 (presynthetic), S (DNA synthesis), G2
(premitotic), and M (mitotic) phases. Quiescent cells that have not
entered the cell cycle are in
the G0 state. Each cell cycle phase is dependent on the proper
activation and completion of the previous ones and the cycle stops
at a place at which an essential gene func-tion is deficient.
Because of its central role in maintaining tissue homeostasis and
regulating physiologic growth processes such as regeneration and
repair, the cell cycle has multiple checkpoints, particularly
during emergence from
G0 into G1 and the transition from G1 to S phase.
Cells can enter G1 either from G0 (quiescent cells) or after
completing mitosis (continuously replicating cells). Quiescent
cells must first go through the transition from G0 to G1, the first
decision step, which functions as a gateway to the cell cycle.
Cells in G1 progress through the cell cycle and reach a critical
stage at the G1-S transition, known as a restriction point, a
rate-limiting step for replication. On
passing this restriction point, normal cells become irreversibly
committed to DNA replication. The cell cycle is tightly controlled
by activators and inhibitors.
• Progression through the cell cycle, particularly at the
G1-S transition, is regulated by proteins called
cyclins, so called because of the cyclic nature of
their production and degradation, and associated enzymes, the
cyclin-dependent kinases (CDKs). CDKs acquire catalytic activity by
binding to and forming complexes with the cyclins.
The orderly progression of cells through the various
phases of the cell cycle is orchestrated by CDKs, which are
activated by binding to the cyclins.
• The CDK–cyclin complexes phosphorylate crucial target proteins
that drive the cell through the cell cycle. On completion of this
task, cyclin levels decline rapidly.
More than 15 cyclins have been identified; cyclins D, E,
A, and B appear sequentially during the cell cycle and
bind to one or more CDKs. The cell cycle may thus be
seen as a relay race in which each leg is regulated by a distinct
set of cyclins: As one set of cyclins leaves the track, the next
set takes over. Activated CDKs
in these complexes drive the cell cycle by phosphorylating proteins
that regulate cell cycle transitions. One such protein is the
retinoblastoma protein (Rb)
• The activity of CDK–cyclin complexes is regulated
by CDK inhibitors (CDKIs), which enforce cell cycle
checkpoints. Embedded in the cell cycle are surveillance mechanisms
that are geared to sensing damage to DNA and chromosomes. These
quality control checks are called checkpoints; they ensure that
cells with damaged DNA or chromosomes do not complete replication.
The G1-S checkpoint monitors the integrity of DNA before DNA
replication, whereas the G2-M checkpoint checks
DNA after replication and monitors whether the cell can safely
enter mitosis. When cells sense DNA damage, checkpoint activation
delays the cell cycle and triggers DNA repair mechanisms. If DNA
damage is too severe to be repaired, the cells are eliminated by
apoptosis, or enter a nonreplicative state called senescence,
primarily through p53-dependent mechanisms, discussed later
on.
Mutations in genes regulating these checkpoints allow
cells with damaged DNA to divide, producing daughter
cells carrying mutations.
• There are several families of CDKIs. One family,
composed of three proteins called p21 (CDKN1A),
p27 (CDKN1B), and p57 (CDKN1C), inhibits the CDKs
broadly, whereas the other family of CDKIs has selective effects on
cyclin CDK4 and cyclin CDK6. The four members of this family—p15
(CDKN2B), p16 (CDKN2A), p18 (CDKN2C), and p19 (CDKN2D)—are
sometimes called INK4 (A to D) proteins.
Alterations in
Cell Cycle
Control Proteins
in Cancer
Cells.
With this background, it is easy to appreciate the mutations
that dysregulate the activity of cyclins and CDKs would favor cell
proliferation. Indeed, all cancers appear to have genetic lesions
that disable the G1-S checkpoint, causing cells to continually
reenter the S phase. For unclear reasons, particular lesions vary
widely in frequency across tumor types.
• Mishaps increasing the expression of cyclin D or CDK4 seem to be
a common event in neoplastic transformation. The cyclin D genes are
overexpressed in many cancers, including those affecting the
breast, esophagus, liver, and a subset of lymphomas and plasma cell
tumors.
Amplification of the CDK4 gene occurs in
melanomas, sarcomas, and glioblastomas. Mutations
affecting cyclins B and E and other CDKs also occur, but they are
much less frequent than those affecting cyclin CDK4.
• The CDKIs frequently are disabled by mutation or gene silencing
in many human malignancies. Germline muta-tions of CDKN2A are
present in 25% of melanoma-prone kindreds. Somatically acquired
deletion or inactivation of CDKN2A is seen in 75% of pancreatic
carcinomas, 40% to 70% of glioblastomas, 50% of esophageal cancers,
and 20% of non–small cell lung carcinomas, soft tissue sarcomas,
and bladder cancers.
A final consideration of importance in a discussion of
growth-promoting signals is that the increased production of
oncoproteins does not by itself lead to sustained proliferation of
cancer cells. There are two built-in mechanisms, cell senescence
and apoptosis, that oppose oncogene-mediated cell growth. So, genes
that regulate these two braking mechanisms must be disabled to
allow unopposed action of oncogenes.